Anti CD63, Monoclonal Antibody (3-13), Fluorescein Conjugated
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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25Tests
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Discontinued
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100Tests
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Document
Overview
This product is a fluorescein-conjugated mouse monoclonal antibody against human CD63 (lysosome-associated membrane glycoprotein 3, LAMP3). This antibody was established by DNA immunization.
CD63 is a four-transmembrane protein and has been used as a marker of extracellular vesicles and activated platelets.
Features
- Detects CD63 with high sensitivity
- Recognizes CD63 expressed on both plasma membranes and extracellular vesicles.
Product Information
| Reactivity* | Human * This antibody does not recognize mouse, rat, or bovine CD63. |
| Storage Buffer | 1 x TBS with Tween 20. Contains stabilizers and preservatives. |
| Host | Mouse |
| Isotype | IgG1 |
| Clone No. | 3-13 |
| Labeling Substance | Fluorescein Excitation: 494 nm, Emission: 521 nm |
*Reactivity confirmation condition
The reactivity of this antibody against the serum of each organism was tested by ELISA.
When the signal value was the same level as the blank, it was judged as "not recognized".
Flow Cytometry Protocols
Experimental conditions, such as sample volume and antibody concentration, will need to be optimized for each assay.
| Cells | Use 10 µL of this product per 1 x 106 cells. |
| Extracellular Vesicles | Use 10 µL of this product for 33 µL or more of cell culture supernatant or body fluid. * Extracellular vesicles are extremely small particles. Thus, prior to analyzing these vesicles by flow cytometry using this product, they will need to be captured on the surface of magnetic beads. We recommend the following product for capturing extracellular vesicles: PS Capture™ Exosome Flow Cytometry Kit (Cat. No. 297-79701) |
Applications
Flow Cytometry
Detection of CD63 on the surface of COLO201 cells
Number of Cells Stained: 1 x 106
COLO201 cells were analyzed by flow cytometry using fluorescein-conjugated anti-CD63 antibodies.
This result indicates that our fluorescein-conjugated anti-CD63 antibody could detect cell surface-expressed CD63 with higher sensitivity than another supplier’s antibody.
Detection of CD63 on the surface of extracellular vesicles in the culture supernatant of COLO201 cells and human serum respectively
Sample Volume (culture supernatant or serum): 33 µL
Extracellular vesicles in culture supernatants of COLO201 cells and human serum, respectively, were isolated using the PS Capture™ Exosome Flow Cytometry Kit. Subsequently, these vesicles were analyzed by flow cytometry using fluorescein-conjugated anti-CD63 antibodies.
These results showed that our fluorescein-conjugated anti-CD63 antibody was superior to another supplier’s antibody in detecting CD63 proteins expressed on the surface of extracellular vesicles.
Overview / Applications
| Outline | Anti human CD63 (lysosome-associated membrane glycoprotein 3: LAMP3) mouse monoclonal antibody was produced by hybridoma, clone No. 3-13. This antibody is labeled with Fluorescein and can be used for flow cytometry. CD63 has the four transmembrane domains, and known as the marker of platelet activation. It also known as the marker of the extracellular vesicle. Excitation : 494 nm, Emission : 521 nm |
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| Purpose | Flow Cytometry |
| Instructions | Flow Cytometric analysis of exosomes: 1 test of product for 33uL~ culture supernatant/biofluids is recommended for the exosome flow cytometry using PS Capture(TM) Exosome Flow Cytometry Kit. (Note)The particle size of exosome is too small to detect directly by Flow Cytometry. To detect exosome, it should be captured by affinity beads for exosome marker. When using Anti CD63, Monoclonal Antibody(3-13), Fluorescein conjugated, please use with the exosome capture beads. Please optimize the most appropriate concentration for your analysis and samples. Flow cytometric analysis of cells: 1 test of product for 1x10^6 cells. |
Property
| Specificity | Human CD63. *Does not react with mouse, rat, and bovine CD63. [Reactivity confirmation condition] The reactivity of these antibodies against the serum of each organisms was measured by ELISA. When the signal value was the same level as the blank, it was judged as "non-reactive. |
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| Composition | 1xTBS aqueous solution with Tween20, the stabilizer, and the preservative. |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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