Anti Iba1 Antibodies

Iba1 (Ionized calcium-binding adapter molecule1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system¹⁾. It is expressed in both resting and activated microglia, but is reportedly expressed more highly in activated microglia²⁾. It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1).

Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis³⁾.

Fujifilm Wako’s "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741), which allows even microglia processes to be stained by immunohistochemical staining, is used by researchers all over the world as a microglia marker antibody standard.

Microglia staining
Anti Iba1, Rabbit
(for immunocytochemistry)

Features of Anti Iba1, Rabbit (for immunocytochemistry)

• Enables even the processes of microglia to be immunohistochemical-stained*
• Relatively small amount needed, with concentrations ranging from 1:500 to 1:1000.

Number of publications

Anti Iba1, Rabbit (for immunocytochemistry) has been appearing in an increasing number of publications, including many articles published in top journals (Nature, Cell, Neuron, etc.), and the annual number of such publications exceeded 1,200 in 2021.

• 

• Journal Name	Number of Publications	Impact Factor in 2020
  Nature	29	49.962
  Cell	31	41.582
  Science	1	47.728
  Nature Medicine	10	53.440
  Nature Neuroscience	49	24.884
  Nature Immunology	6	25.606
  Nature Biotechnology	4	54.908
  Nature Methods	2	28.547
  Neuron	29	17.173
  Total	161

Selection Flowchart

Product Lineup

Immunohistochemistry

▼Anti Iba1, Rabbit (for immunocytochemistry)

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1000
[Data by courtesy of]
Dr. Sanagi, Dr. Manabe,
Dr. Ichinohe, Dr. Kohsaka
National Center of
Neurology and Psychiatry

Species: Mouse
Site: Cerebellum
Sample: Frozen section
Antibody concentration: 1:1000

▼Anti Iba1, Rabbit (for paraffin section)

Species: Rat
Site: Hippocampus vicinity
Sample: Paraffin section
Antibody concentration: 1:1000

▼Anti Iba1, Rabbit, Biotin-conjugated

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200
[Data by courtesy of]
Dr. Sanagi, Dr. Manabe,
Dr. Ichinohe, Dr. Kohsaka
National Center of
Neurology and Psychiatry

▼Anti Iba1, Rabbit, SPICA Dye™ 568-conjugated

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200

▼Anti Iba1, Rabbit, SPICA Dye™ 594-conjugated

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200

▼Anti Iba1, Rabbit, Red Fluorochrome (635)-conjugated

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:200
[Data by courtesy of]
Dr. Sanagi, Dr. Manabe,
Dr. Ichinohe, Dr. Kohsaka
National Center of
Neurology and Psychiatry

▼Anti Iba1, Goat

Species: Mouse
Site: Hippocampus
Antibody concentration: 1:200
[Data by courtesy of]
Dr. Takata
Division of Integrated
Pharmaceutical Sciences,
Kyoto Pharmaceutical University

▼Anti Iba1, Mouse Monoclonal Antibody (NCNP24)

Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1:1000
[Data by courtesy of]
Dr. Sanagi, Dr. Manabe,
Dr. Ichinohe, Dr. Kohsaka
National Center of
Neurology and Psychiatry

Western blotting

▼Anti Iba1, Rabbit (for western blotting)

　1.Iba1 protein...10 ng
　2.Rat Microglia...10 μg
　3.Rat Neuron...10 μg
　4.Rat cerebral cortex...150 μg

SDS-PAGE: 5.5% stacking gel, 12.5% running gel, 100V
Blocking: 3% skim milk/TBS, 1 hour, room temperature.
Primary antibody: 1:1000 concentration, 3% skim milk/TTBS, overnight, 4℃
Secondary antibody: Peroxidase-labeled Anti Rabbit IgG (1/5000), 3% skim milk/TTBS, 1 hour, room temperature

▼Anti Iba1, Goat

　1.Rat primary culture microglia...10 μg
　2.Rat primary culture neuron...10 μg
　3.Rat primary culture astrocyte...10 μg
　4.Rate cerebral cortex...100 μg

Primary antibody: Anti Iba1, Goat, 1:1000
Secondary antibody: Anti Goat IgG, HRP-conjugated

1. Imai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S.: Biochemical and biophysical research communications, 224(3), 855(1996).
   A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage
2. Mori, I., Imai, Y., Kohsaka, S., & Kimura, Y.: Microbiology and immunology, 44(8), 729(2000).
   Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection
3. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S., & Imai, Y.: Biochemical and biophysical research communications, 286(2), 292(2001).
   Iba1 is an actin-cross-linking protein in macrophages/microglia.
4. Ahn, J.H., et al.: Lab. Anim. Res., 28(3), 165 (2012).
   Comparison of alpha-synuclein immunoreactivity in the spinal cord between the adult and aged beagle dog
5. Ide, T., et al.: J. Vet. Med .Sci., 72(1), 99 (2010).
   Histiocytic Sarcoma in the Brain of a Cat
6. Gaige, S., et al.: Neurotoxicology, 34, 135(2013).
   c-Fos immunoreactivity in the pig brain following deoxynivalenol intoxication: Focus on NUCB2/nesfatin-1 expressing neurons
7. Rodriguez-Callejas, J.D. et al.: Front. Aging Neurosci., 8, 315(2016).
   Evidence of Tau Hyperphosphorylation and Dystrophic Microglia in the Common Marmoset
8. Fantin, A., et al.: Blood, 116(5), 829(2010).
   Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction