LBIS (TM) Mouse IL-17A ELISA Kit
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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96Tests
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Kit component
96 tests
| Antibody-coated Plate | 96 wells/1 plate |
|---|---|
| Mouse IL-17A Standard | 1 bottle |
| Buffer | 60 mL/1 bottle |
| Biotin-conjugated Antibody | 1 bottle |
| Peroxidase-conjugated Streptavidin Solution | 100 μL/1 bottle |
| TMB Solution | 12 mL/1 bottle |
| Stop Solution | 12 mL/1 bottle |
| Wash Solution (10x) | 100 mL/1 bottle |
| Plate Seal | 4 sheets |
Product Overview
Interleukin 17A (IL-17A) is an inflammatory cytokine produced primarily by immune cells including Th17 cells. It acts on a diverse range of cells ― including macrophages, fibroblasts, epithelial cells, endothelial cells, and stromal cells ― and efficiently induces the production of inflammatory mediators in these cells.
LBIS™ Mouse IL-17A ELISA Kit is a sandwich ELISA kit for the quantitative measurement of mouse IL-17A. It enables highly sensitive quantification of IL-17A in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.
Kit Performance
| Assay target | IL-17A |
|---|---|
| Calibration curve range | 2.06 – 800 pg/mL |
| Analysis sample | Mouse Serum/Plasma (EDTA, heparin) |
| Sample amount | 25 μL (2x dilution) |
| Measurement duration | Approx 3 hours and 50 minutes |
| Wavelength | Primary wavelength : 450 nm Reference wavelength : 620 nm |
検量線例
Data
Repeatability (within run precision)
Repeatability was conducted by measuring fivefold assays of mouse serum samples at two concentrations.
| n＼ID | ID1 (pg/mL) | ID2 (pg/mL) |
|---|---|---|
| 1 | 261 | 18.6 |
| 2 | 248 | 17.8 |
| 3 | 243 | 18.4 |
| 4 | 264 | 18.2 |
| 5 | 274 | 19.2 |
| mean | 258 | 18.4 |
| SD | 12.5 | 0.52 |
| CV(%) | 4.8 | 2.8 |
- [Result]
- The CV (%) of mouse serum was 2.8-4.8%, indicating good repeatability.
Reproducibility (between run precision)
Reproducibility was conducted by measuring mouse serum samples at three concentrations each for four days.
| Day＼ID | ID3 (pg/mL) | ID4 (pg/mL) | ID5 (pg/mL) |
|---|---|---|---|
| 1 | 281 | 40.6 | 5.92 |
| 2 | 309 | 39.4 | 5.65 |
| 3 | 308 | 41.2 | 6.11 |
| 4 | 297 | 41.8 | 5.98 |
| mean | 299 | 40.8 | 5.92 |
| SD | 13.0 | 1.0 | 0.19 |
| CV(%) | 4.4 | 2.5 | 3.3 |
- [Result]
- The CV (%) of mouse serum was 2.5-4.4%, indicating good reproducibility.
Dilution Linearity Test
Mouse serum and plasma (EDTA/heparin) were serially diluted twofold using the provided buffer and measured in duplicate to evaluate linearity.
Mouse serum/plasma (EDTA, heparin)
- [Result]
- All measurement results showed good linearity within the measurement range.
Spike and Recovery Test
Standard solutions at three concentrations were added to mouse serum/plasma (EDTA/heparin) were conducted, with each measurement performed in duplicate.
Mouse serum
| Spiked amount (pg/mL) |
Measurement value (pg/mL) |
Recovery amount (pg/mL) |
Recovery (%) |
|---|---|---|---|
| - | 1.41 | - | - |
| 10 | 11.2 | 9.79 | 97.9 |
| 50 | 49.1 | 47.7 | 95.4 |
| 250 | 264 | 263 | 105 |
| mean | 99.4 | ||
Mouse plasma (EDTA)
| Spiked amount (pg/mL) |
Measurement value (pg/mL) |
Recovery amount (pg/mL) |
Recovery (%) |
|---|---|---|---|
| - | 0.328 | - | - |
| 10 | 9.99 | 9.66 | 96.6 |
| 50 | 50.6 | 50.3 | 101 |
| 250 | 257 | 257 | 103 |
| mean | 100 | ||
Mouse plasma (heparin)
| Spiked amount (pg/mL) |
Measurement value (pg/mL) |
Recovery amount (pg/mL) |
Recovery (%) |
|---|---|---|---|
| - | 0.749 | - | - |
| 10 | 10.9 | 10.2 | 102 |
| 50 | 54.6 | 53.9 | 108 |
| 250 | 264 | 263 | 105 |
| mean | 105 | ||
- [Result]
- Good recovery rates were confirmed.
Example of Sample Measurement
Mouse serum and plasma (EDTA/heparin) samples were measured and compared using this kit and competitor Company A.
Mouse serum
(BALB/c, male, 8-week-old, 16-hour fast)
| ID＼n | Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
|---|---|---|
| 1 | 5.75 | N.D. |
| 2 | 46.6 | N.D. |
| 3 | 45.2 | 5.14 |
| 4 | 1.90 | N.D. |
| 5 | 53.2 | N.D. |
Mouse plasma (EDTA)
(BALB/c, male, 8-week-old, 16-hour fast)
| ID＼n | Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
|---|---|---|
| 1 | 96.8 | N.D. |
| 2 | 4.28 | N.D. |
| 3 | 12.4 | N.D. |
| 4 | 3.01 | N.D. |
| 5 | 5.62 | 5.21 |
Mouse plasma (heparin)
BALB/c, male, 8-week-old, 16-hour fast
| ID＼n | Fujifilm Wako (pg/mL) |
Company A (pg/mL) |
|---|---|---|
| 1 | 57.5 | N.D. |
| 2 | 26.7 | N.D. |
| 3 | 47.2 | N.D. |
| 4 | 6.88 | N.D. |
| 5 | 18.2 | N.D. |
N.D.: Below the lower limit of quantification
Red: Values outside the quantification range; shown as reference
- [Result]
- This product was able to detect IL-17A even in samples that were below the lower limit of quantification of another manufacturer's kit.
FAQ
About samples
- What anticoagulants should I use?
- We recommend using EDTA or heparin as anticoagulant.
- How should I store and prepare the samples?
- Samples should be immediately assayed or stored below -35℃ until assay. Before starting assay, shake thawed samples sufficiently. Do not repeat freeze-and-thaw cycles. Dilution of a sample should be made in a PP or PE test tube using the provided buffer prior to adding them to wells.
About kit usage
- What instruments, and equipment are required for the assay using this kit?
- The instruments and equipment required for the use of this kit are listed below;
・Deionized water (or distilled water)
・Test tubes for preparation of standard solution series
・Glassware for dilution of Wash Solution (10x) (a graduated cylinder, a bottle)
・Pipettes (disposable tip type). One should be able to deliver 50 μL precisely, and another for 100 – 1,000 μL
・Syringe-type repeating dispenser like Eppendorf ultipette plus which can dispense 100 μL
・Paper towel to remove washing buffer remaining in wells
・A vortex-type mixer
・A shaker for 96 well-plate (600 – 1,200 rpm)
・An automatic washer for 96 well-plate (if available), or a wash bottle with a jet nozzle
・A 96 well-plate reader (450 ± 10 nm, 620 nm :600 - 650 nm)
・Software for data analysis
- How should I use the mouse IL-17A standard?
- The amount of purified water to be added varies by lot. Please reconstitute the mouse IL-17A standard with the amount of purified water specified in the “Reconstitution of standard” section on this product page to prepare the original standard solution (8 ng/mL). Then, dilute the solution using the kit-provided buffer that has been equilibrated to room temperature.
- What are the storage conditions for each reagent?
- Buffer & TMB Solution
Use only volume you need for your assay. Remaining reagents should be stored at 2-10℃ closing the cap tightly. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Stop Solution
Close the cap tightly and store at 2-10℃.
Mouse IL-17A Standard & Biotin-conjugated Antibody
The reconstituted standard solution (original standard solution) should be used within 2 weeks if stored in a refrigerator. Dispose the remaining diluted standard solutions after use.
Peroxidase-conjugated Streptavidin Solution
Remaining working solution (already diluted) should be disposed. The rest of the undiluted solution (unused): close the cap tightly and store at 2-10℃. Once opened, we recommend using as soon as possible to avoid influence by environmental condition.
Prepare reagent solutions in appropriate volume for your assay.
Overview / Applications
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Manufacturer Information
Alias
- 637-44711 (Previous Code)
AKMIL17-011 (Previous Code)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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