Human s-IgA ELISA Kit Wako
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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96Tests
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In stock in Japan |
※Check availability in the US with the distributor.
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Kit component
Kit Component
| Antibody-coated Plate | 1 plate |
|---|---|
| s-IgA Standard | 1 vial |
| Anti-Human s-IgA Antibody, HRP-conjugated | 12 mL |
| TMB Solution | 12 mL |
| Stop Solution | 12 mL |
| Buffer (2×) | 25 mL |
| Wash Solution (20×) | 50 mL |
| Plate Seal | 3 sheets |
Product Information
Secretory IgA (s-IgA) is an IgA to which a secretory component (SC) is bound, basically occurring as a dimer but also as tetramer, etc. IgA in secretions occurs principally as s-IgA, which plays an important role in protection from infection on the mucosa of the respiratory and gastrointestinal tracts. s-IgA is also found in human saliva and produced in plasma cells in the salivary glands. The level of s-IgA in human saliva is reportedly increased by acute stress1) and decreased by chronic stress2) 3), giving it utility as a stress biomarker.
Human s-IgA ELISA Kit Wako is an ELISA kit for assaying s-IgA in human saliva.
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Kit Performance
The range of the calibration curve 0.082 - 20 µg/mL Assay target Secretory IgA (s-IgA) Sample Human Saliva The sample amount required 5 µL Assay time 2.5 hours Detection method Colorimetry Within assay variation CV < 6％ Between assay variation CV < 12％ -
Example of Calibration Curve
Data
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Spiked recovery test
Sample Amount spiked(µg/mL) Measured value(µg/mL) Recovery rate(%) Saliva１ 0 64.8 - 40 108.8 103.8 160 220.8 98.2 400 404.8 87.1 Saliva 2 0 46.4 - 40 96.0 111.1 160 196.0 95.0 400 432.0 96.8 Saliva 3 0 120.8 - 40 189.6 117.9 160 274.4 97.7 400 476.0 91.4 Saliva 4 0 112.0 - 40 157.6 103.7 160 244.8 90.0 400 441.6 86.3 Saliva 5 0 105.6 - 40 152.0 104.4 160 250.4 94.3 400 600.8 118.8 -
Dilution linearity test
Sample Dilution Measured value(µg/mL) Saliva１ 1/1 363.5 1/2 201.7 1/4 107.7 1/8 53.6 Saliva 2 1/1 237.4 1/2 123.9 1/4 65.3 1/8 32.5 Saliva 3 1/1 310.0 1/2 167.3 1/4 89.5 1/8 42.7 Saliva 4 1/1 293.3 1/2 145.3 1/4 74.7 1/8 38.3 Saliva 5 1/1 188.2 1/2 97.5 1/4 47.4 1/8 26.8
Cross reactivity with various types of immunoglobulins
| Immunoglobulins | Cross-reactivity(%) |
|---|---|
| Serum IgG(Human) | < 0.1 |
| Serum IgA(Human) | 0.3 |
| Serum IgM(Human) | 0.0 |
| Serum IgE(Human) | 0.0 |
References
- Viena, T. D. et al.: Biol. Psychol., 91(2), 307(2012).
Differential effects of mild chronic stress on cortisol and S-IgA responses to an acute stressor - Deinzer, R. et al.: Int. J. Psychophysiol., 37(3), 219(2000).
Prolonged reduction of salivary immunoglobulin A (sIgA) after a major academic exam - Phillips, A. C. et al.: Brain, behav. Immun., 20(2), 191(2006).
Stressful life events are associated with low secretion rates of immunoglobulin A in saliva in the middle aged and elderly
FAQ
About sample
- How should I store samples?
- Saliva samples should be stored frozen at -30°C or below. Avoid repeated freezing and thawing of samples.
- How should I prepare samples?
- Dilute the saliva sample 80-fold with a buffer previously diluted with distilled water (395 μL of diluted buffer : 5 μL of saliva sample). If the saliva sample is stored frozen, thaw at room temperature and stir well. If the concentration of the diluted sample is higher than 20 μg/mL, further dilute the 80-fold diluted sample with the buffer before measurement.
- How should I pretreat highly viscous saliva samples?
- Highly viscous saliva may not provide a correct measurement value. Precipitate mucin, etc. by centrifugation and use the supernatant.
About analyte
- What is the degree of cross-reactivity with non-human species?
- Fujifilm Wako has not checked cross-reactivity with non-human species (mice, rats, etc.).
About the kit
- What is the origin of the standard?
- The origin is a purified product from human colostrum.
- Can I divide plates?
- Since the plate in this kit are separable and each row (8 wells) is detachable, divided use of the kit is possible.
About kit usage
- What reagents, instruments, and equipment are required for measurement with this kit?
- The following reagents, instruments, and equipment are required to use this kit:
- Micropipettes and disposable tips (5 to 1,000μL) (8- or 12- channel pipettes are recommended.)
- Microplate reader which can read extinction 3.0 at 450 nm
- Microplate shaker or conventional shaker
- Test tubes for dilution of standard solutions and samples (glass or polypropylene)
- Microplate washer （For manual operation, a continuous dispenser, needle dispenser, and an aspirator or vacuum pump are recommended.）
- Graduated cylinder (1,000 mL)
- Distilled or deionized water
Overview / Applications
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Manufacturer Information
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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