CD63-Capture Human Exosome ELISA Kit (Streptavidin HRP)
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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96Tests
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In stock in Japan |
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Document
Kit component
Kit component
| Anti-CD63 Antibody-immobilized 96 Well Plate | 8 well x 12 strips / 1 plate |
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| Plate Seal | 4 sheets |
| Sample Reaction Buffer | 50 mL x 1 |
| Antibody Reaction Buffer | 50 mL x 1 |
| Washing Buffer (10x) | 100 mL x 1 |
| Control Biotinylated Antibody Anti-CD63 (100x) | 120 μL x 1 |
| HRP-conjugated Streptavidin (100x) | 240 μL x 1 |
| TMB Solution | 12 mL x 1 |
| Stop Solution | 12 mL x 1 |
[Reference data] The detection sensitivity of COLO201 cell-derived extracellular vesicles
A standard curve was prepared using Exosome, from COLO201 cells, purified [code No. 052-09301] . The detection limit and the quantification limit of this kit were calculated using the standard curve. Each standard was measured at n = 6 and Blank was measured at n = 12.
Sample Preparation
Sample Dilution
Dilute samples at least twice with Sample Reaction Buffer included in the kit so that the absorbance value at 450 nm (value obtained by subtracting the absorbance at a secondary wavelength of 620 nm) is in the range of 0.2 to 2.5 [See "Typical Data of Kit" 4].
Consider the optimum dilution rate for the sample before performing this measurement.
Removal of microvesicles and apoptotic vesicles
Even though CD9, CD63, and CD81 have been identified as exosome markers, when performing ELISA strictly targeting exosomes, pretreatment by centrifugation should be performed to remove extracellular vesicles other than exosomes.
Pretreatment procedure by centrifugation
Blood Sample [serum, plasma, etc.) (Before dilutiong Sample Reaction Buffer)
↓ 10,000 x g, 30min., 4℃
Collect the supernatant
↓
To the sample dilution step.
Overview / Applications
| Kit Components | Anti-CD63 Antibody-immobilized 96 Well Plate 1plate, Plate Seal 4sheets, Sample Reaction Buffer 50mL, Antibody Reaction Buffer 50mL, Washing Buffer (10x) 100mL, Control Biotinylated Antibody Anti CD63(100x) 120uL, HRP-conjugated Streptavidin(100x) 240uL, TMB Solution 12mL, Stop Solution 12mL |
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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