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CD81-Capture Human Exosome ELISA Kit (Streptavidin HRP)

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
292-83801
Barcode No
4548995084804
96Tests
List Price
990.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

Kit component

Anti-CD81 Antibody-immobilized 96 Well Plate 8 well x 12 strips / 1 plate
Plate Seal 4 sheets
Sample Reaction Buffer 50 mL x 1
Antibody Reaction Buffer 50 mL x 1
Washing Buffer (10x) 100 mL x 1
Control Biotinylated Antibody Anti-CD81 (100x) 120 μL x 1
HRP-conjugated Streptavidin (100x) 240 μL x 1
TMB Solution 12 mL x 1
Stop Solution 12 mL x 1

[Reference data] The detection sensitivity of COLO201 cell-derived extracellular vesicles

A standard curve was prepared using Exosome, from COLO201 cells, purified [code No. 052-09301]. The detection limit and the quantification limit of this kit were calculated using the standard curve. Each standard was measured at n = 6 and Blank was measured at n = 12.

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Sample Preparation

Sample Dilution

Dilute samples at least twice with Sample Reaction Buffer included in the kit so that the absorbance value at 450 nm (value obtained by subtracting the absorbance at a secondary wavelength of 620 nm) is in the range of 0.2 to 2.5 [See "Typical Data of Kit" 4.].
Consider the optimum dilution rate for the sample before performing this measurement.

Removal of microvesicles and apoptotic vesicles

Even though CD9, CD63, and CD81 have been identified as exosome markers, when performing ELISA strictly targeting exosomes, pretreatment by centrifugation should be performed to remove extracellular vesicles other than exosomes.

Pretreatment procedure by centrifugation

Blood Sample [serum, plasma, etc.) (Before dilutiong Sample Reaction Buffer)
 ↓ 10,000 x g, 30min., 4℃
Collect the supernatant
 ↓
To the sample dilution step.

FAQ

Control Biotinylated Antibody Anti-CD81 (100x)

Overview / Applications

Kit Components Anti-CD81 Antibody-immobilized 96 Well Plate 1plate, Plate Seal 4sheets, Sample Reaction Buffer 50mL, Antibody Reaction Buffer 50mL, Washing Buffer (10x) 100mL, Control Biotinylated Antibody Anti CD81(100x) 120uL, HRP-conjugated Streptavidin(100x) 240uL, TMB Solution 12mL, Stop Solution 12mL

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Manufacturer Information

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Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

877-714-1920

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