This site may not be displayed properly in the browser being used.
We recommend that you update to the latest browser.

It has been added to the cart.

It has been added to the comparison table.

Detail Information
  1. Top
  2. Life Science
  3. Genetics
  4. microRNA
  5. microRNA Isolation Kit Series
  6. MagCapture(TM) microRNA Isolation Kit, Mouse Ago2

MagCapture(TM) microRNA Isolation Kit, Mouse Ago2

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Close
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
297-74201
Barcode No
4548995029881
10Tests
List Price
616.00 USD

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Recovering microRNA from mouse cell strains

We used MagCapture™ microRNA Isolation Kit, Mouse Ago2 and a previous product to obtain microRNA from mouse P388D1 cells (2×107 cells), then compared quantities of microRNA obtained with denaturing polyacrylamide gel electrophoresis (silver staining) (Fig. 1) and quantitative PCR (miR92a quantity) (Fig. 2). Results showed that the two products yielded equivalent quantities of microRNA.

01842191_img01-R.png

Recovering free Ago2-bound microRNA from rat plasma

01842191_img02-R.png

We used MagCapture™ microRNA Isolation Kit, Mouse Ago2 and a previous product to obtain microRNA from 200 μL of rat plasma, then compared quantities obtained with Quantitative PCR (miR92a quantity). As shown below, using the spin column method with the MagCapture™ resulted in approximately equivalent yield to our previous product, while the one-step method demonstrated greater quantities of microRNA recovered compared to our previous product.

Overview / Applications

Outline This product is for research use only. Do not administer it to human.


This kit utilizes immunoprecipitation methods using an anti mouse Ago2 antibody to easily prepare Ago2-bound microRNAs and their target mRNAs from mouse cells and tissue samples. In addition, Ago2-bound microRNAs present in the extracellular fluid such as plasma, serum, and cell culture supernatant samples can be also prepared in the same way.
[Features]
•High-efficient purification of microRNAs
•Adoption of anti-mouse Ago2 antibody-immobilized magnetic beads
•In a microRNA purification step, either the high-yield one-step method or the spin-column method yielding high-purity microRNAs is selectable
•Setting of an optimized protocol each for samples of cultured cells and tissue, and samples of plasma, serum, and culture supernatant.

Property

Manufacturer Information

Alias

  • microRNA Isolation
    microRNA

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

Ask for help