microRNA ExtractorR SP Kit
- for Genetic Research
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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50Tests
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In stock in Japan |
※Check availability in the US with the distributor.
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Kit component
Kit contents
| Lysis Solution | 20 mL x 1 |
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| Reducing reagent | 60 μL x 1 |
| Protease Solution | 600 μL x 1 |
| Enhancer | 600 μL x 1 |
| Washing Solution 1 | 12 mL x 1 |
| Washing Solution 2 | 22.5 mL x 1 |
| Elution Solution | 4 mL x 1 |
| Spin column/2 mL tube | 50 sets |
Overview
This kit is for extraction of microRNA from serum or plasma of humans and animals. It enables microRNA extraction without use of phenol, chloroform, and other hazardous reagents required in previous methods.
Protocol Flow
Note
The following necessary reagents are not included.
1-Butanol (code No.022-16035)
2-Propanol (code No.168-21675)
Ethanol (code No.054-07225)
For plasma coagulation, please use EDTA or citric acid. Using heparin inhibit PCR detection after RNA extraction.
Application Data
Extraction from normal human plasma
Higher extraction rates observed than from previous methods.
Extraction from rat plasma and serum
Extraction from animal experiments possible.
Recovery rate and reproducibility
High reproducibility exhibited.
Overview / Applications
| Outline | microRNA Extractor SP Kit is designed for the extraction of total RNA including microRNA from serum and plasma of human and rodents(rat, mouse etc.). The extraction method of this kit is based on the procedure using chaotropic reagent, which allows for a safe extraction without hazardous phenol/chloroform, and spin column. This kit is useful microRNA extraction kit for circulating microRNA in serum and plasma. |
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Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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