PTC-Amino Acids Mobile Phase A

for Analysis of PTC-amino Acids

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Protect from light.

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Comparison	Product Number	Package Size	Price	Inventory
	Distributor 163-14971 Barcode No 4987481318403	1L	List Price 95.00 USD	In stock in Japan	Certificate of Analysis

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Application

Overview / Applications

Outline	Analysis of amino acid composition is an important analysis in a variety of applications and fields including protein/peptide research, food analysis and diagnosis of metabolic disorders. The PTC method, an amino acid analysis method, involves labeling with phenyl isothiocyanate (PITC), which has long been used as the Edman reagent, and is superior to other prelabeling methods in that it reacts with secondary amine and the molecular extinction coefficient for the standard compound is similar among any amino acids; however, the analysis requires complex gradients.Our company has completed a system consisting of a Wakopak WS-PTC column and a dedicated eluent allowing for analysis in short periods under simple eluting conditions. This system is applicable to any general-purpose HPLC systems supplied by other companies with few alterations in eluting conditions, and enables high-sensitivity, highly reproducible PTC amino acid analysis in short periods without troublesome consideration of conditions.

Outline

Analysis of amino acid composition is an important analysis in a variety of applications and fields including protein/peptide research, food analysis and diagnosis of metabolic disorders. The PTC method, an amino acid analysis method, involves labeling with phenyl isothiocyanate (PITC), which has long been used as the Edman reagent, and is superior to other prelabeling methods in that it reacts with secondary amine and the molecular extinction coefficient for the standard compound is similar among any amino acids; however, the analysis requires complex gradients.Our company has completed a system consisting of a Wakopak WS-PTC column and a dedicated eluent allowing for analysis in short periods under simple eluting conditions. This system is applicable to any general-purpose HPLC systems supplied by other companies with few alterations in eluting conditions, and enables high-sensitivity, highly reproducible PTC amino acid analysis in short periods without troublesome consideration of conditions.

Property

Appearance	Colorless clear liquid
pH	pH (25 degrees C) : 6.0 - 6.2
Flash Point	None
Density	0.989 - 0.999g/ml (20 degrees C)
Refractive Index	1.333 - 1.339(20 degrees C/D)

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Product List

Product Name
Wakopak Wakosil-PTC 4.0*150mm
Wakopak Wakosil-PTC 4.0*200mm
Wakopak Wakosil-PTC 4.0*250mm
PTC-Amino Acids Mobile Phase B	for Analysis of PTC-amino Acids
Phenyl Isothiocyanate	for Amino Acid Sequence Analysis
Triethylamine	Wako Special Grade
Ethanol (99.5)	for HPLC
20% Hydrochloric Acid	Super Special Grade
Wakopak® Wakosil PTH-II φ4.6mm × 250mm (S-PSQ)
PTH-amino Acids Mobile Phase	for SHIMADZU Protein Peptide Sequencer
Wakopak® Wakosil PTH-GR (S-PSQ)(for Gradient Elution)
PTH-amino Acids Mobile Phase A (for Gradient Elution)	for SHIMADZU Protein Peptide Sequencer
PTH-amino Acids Mobile Phase B (for Gradient Elution)	for SHIMADZU Protein Peptide Sequencer
APDSTAG Wako Eluent	for Amino Acid Automated Analysis
Acetonitrile	for LC/MS

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