Anti V5 tag, Monoclonal Antibody(6F5)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -20 degrees C.
- Host :
- Mouse
- Clone No. :
- 6F5
- Classification :
- Monoclonal
- GHS :
-
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- Structural Formula
- Label
- Packing
- SDS
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Comparison
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Certificate of Analysis
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1mg
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Out of Stock |
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5mg
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Discontinued
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200ug
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Application
Overview / Applications
| Outline | The GKPIPNPLLGLDST peptide derived from Simian Virus 5 was consisted of 14 amino acids and has been used as the tag for detection and/or purification of recombinant protein. Monoclonal anti V5 antibody was produced by hybridoma clone 6F5. This clone was established by immunization of synthetic GKPIPNPLLGLDST peptide which was coupled with keyhole limpet hemocyanin (KLH) to mouse. This antibody was purified by affinity chromatography on Protein A from mouse ascites. This product is for laboratory use only ; use in any such application is the responsibility of the user. Working Dilution: Western Blot (1:1,000 to 1:10,000), immunoprecipitation (2 to 10 μg/20 μL 10% Protein A or Protein G beads slurry). Subclass: IgG2b Antigen: synthetic GKPIPNPLLGLDST peptide which was coupled with keyhole limpet hemocyanin (KLH) |
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| Instructions | Western blot 1:1000-1:10000 Immunoprecipitation 2-10ug/ 20uL 10% Protein A or Protein G beads slurry |
Property
| Appearance | Liquid |
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| Concentration | 0.4 - 0.6mg/ml Protein |
Antibody Information
| Antigen | V5 tag |
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| Antigen Alias | V5, Epitope V5-tag V5 tag (V5 epitope tag) V5 epitope tag |
| Subclass | IgG2b |
Manufacturer Information
Alias
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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