Anti Green Fluorescent Protein, Monoclonal Antibody(mFX75)

for Immunochemistry

Manufacturer :: FUJIFILM Wako Pure Chemical Corporation

Storage Condition :: Keep at -20 degrees C.

Source :: Aequorea victoria

Host :: Mouse
Clone No. :: mFX75

Classification :: Monoclonal

Citations (14)

PubMed Central^® search

Structural Formula
Label
Packing

SDS

Comparison	Product Number	Package Size	Price	Availability	Certificate of Analysis	Purchase
	Distributor 012-22541 Barcode No 4987481489677	100uL	List Price 387.00 USD	In stock in Japan	Certificate of Analysis	Distributor

※Check availability in the US with the distributor.

Document
Application
Overview / Applications
Property
Antibody Information
Manufacturer Information
Alias
Related Information

Document

SDS

Product Specification Sheet

Package Insert

100uL

Spectral Data

Certificate of Analysis

100uL

Calibration Certificate

Analytical Charts

Application

Overview / Applications

Outline	This product is for research use only. Do not administer it to human. Green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice. Wako offers a mouse monoclonal antibody for Western blotting of GFP. [Concentration] Displayed on label. [Storage Buffer] D-PBS(-), 10 mmol/L Hepes (pH 7.4) and 10 mmol/L Sodium Azide. [Preparation] This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP's partial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by Hollow fiber instrument was purified by Protein A affinity chromatography and prepared in D-PBS(-) solution containing 10 mmol Hepes (pH 7.4) and 10 mmol/L NaN₃. [Clone No.] mFX75 [Subclass] IgG_2a·κ [Working Dilution] Western blotting 1:500 - 1:1,000 [Reference] 1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G. and Cormier, M. J .: Gene, 111, 229 (1992).
Purpose	Western blotting
Instructions	Working Dilution 1 : 500 - 1 : 1,000

Outline

This product is for research use only. Do not administer it to human.

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice. Wako offers a mouse monoclonal antibody for Western blotting of GFP.

[Concentration]
Displayed on label.

[Storage Buffer]
D-PBS(-), 10 mmol/L Hepes (pH 7.4) and 10 mmol/L Sodium Azide.

[Preparation]
This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP's partial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by Hollow fiber instrument was purified by Protein A affinity chromatography and prepared in D-PBS(-) solution containing 10 mmol Hepes (pH 7.4) and 10 mmol/L NaN₃.

[Clone No.]
mFX75

[Subclass]
IgG_2a·κ

[Working Dilution]
Western blotting 1:500 - 1:1,000

[Reference]
1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G. and Cormier, M. J .: Gene, 111, 229 (1992).

Purpose

Western blotting

Instructions

Working Dilution 1 : 500 - 1 : 1,000

Property

Appearance	Liquid

Antibody Information

Antigen	Green Fluorescent Protein
Antigen Alias	Green Fluorescent Protein GFP Green Fluorescent Protein(GFP) GFP (Green Fluorescent Protein)

Manufacturer Information

Alias

Anti GFP, MoAb(mFX75)

Related Information

Product List

Product Name
Anti Green Fluorescent Protein, Monoclonal Antibody	for Genetic Research

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.