Anti Green Fluorescent Protein, Monoclonal Antibody

Concentration: Shown on label, phosphate-buffered saline (PBS), pH 7.4 containing 10 mmol/L HEPES and 10 mmol/L sodium azide.Preparation: This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP'spartial peptide coupled with BSA after being immunized with GFP. The hybridoma supernatant cultured by protein free culture was subjected to ultrafiltration with an ultra-filter 30,000-molecular-weight cutoff and prepared in PBS solution containing 10 mmol/L HEPES and 10 mmol/L sodiumazide.

Clone No.: mFX73Subclass: IgG2a.kSpecificity: Specific for Native GFP and its variant from jellyfish Aequorea victria. Denatured protein lacks antigen determinants.Working Concentration: Immunoprecipitation 1-5 micrograms / 5 microliter Sepharose Immunohistochemistry 0.5-1 micrograms / mL

References:

1. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G. and Cormier, M. J.: Gene, 111, 229 (1992).
2. Zhen, M. and Jin, Y. : Nature, 401, 371 (1999).
3. Nakagawa, M., Fukata, M., Yamaga, M., Itoh, N. and Kaibuchi, K. : J. Cell Sci., 114, 1829 (2001).
4. Fukata, M., Nakagawa, M., Itoh, N., Kawajiri, A., Yamaga, M., Kuroda, S. and Kaibuchi, K. : Mol. Cell Biol., 21, 2165 (2001).