IL-6 ELISA Kits

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that induces the differentiation of B cells into antibody-producing cells and plays a broad and important role in immune responses, inflammatory reactions, and antibody production. IL-6 is produced and secreted by a variety of cell types, including T cells, monocytes, fibroblasts, keratinocytes, and endothelial cells. At the cell surface, IL-6 binds to a receptor complex comprising IL-6 receptor α (IL-6Rα) and the co-receptor gp130, triggering downstream signaling. A well-known downstream signaling pathway of IL-6 is the IL-6/JAK2/STAT3 pathway, in which JAK2 binds to tyrosine residues on gp130 and activates STAT3 in the cytoplasm, leading to transcriptional activation of various gene clusters¹⁾.
IL-6 is also implicated in the pathogenesis of autoimmune diseases such as rheumatoid arthritis, inflammatory conditions such as gout, and cytokine storms leading to COVID-19-associated acute respiratory distress syndrome (ARDS). In rheumatoid arthritis, IL-6 exerts a wide range of effects, including T cell activation, production of rheumatoid factor and anti-CCP antibodies (ACPA), increased CRP, anemia and thrombocytosis, osteoclast differentiation, cartilage destruction via MMP production, and synovial angiogenesis via VEGF production²⁾. In COVID-19-associated cytokine storms, the cooperative action of the transcription factors NF-κB and STAT3 activates the IL-6 amplification circuit, driving excessive production of pro-inflammatory cytokines. The IL-6/STAT3 pathway has consequently gained attention as a therapeutic target³⁾.

LBIS™ Human IL-6 ELISA Kit is a sandwich ELISA kit for the quantitative measurement of human IL-6. This kit enables highly sensitive quantification of IL-6 in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Kit Performance

Assay target	IL-6
Calibration curve range	1.16 - 500 pg/mL
Analysis sample	Human Serum/Plasma (EDTA・Heparin) /Cell culture supernatant*
Sample volume	50 μL (2x dilution)
Measurement duration	Approx. 3 hours and 50 minutes
Wavelength	Primary wavelength : 450 nm Reference wavelength : 620 nm

• Measurement availability depends on the culture medium.
  When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.

Example of Calibration Curve

Measurement of IL-6 in Human Serum

[Result]
This product was able to detect IL-6 even in samples that were below the lower limit of quantification of another manufacturer's kit.

LBIS™ Mouse IL-6 ELISA Kit is a sandwich ELISA kit for the quantitative measurement of human IL-6. This kit enables highly sensitive quantification of IL-6 in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Kit Performance

Assay target	IL-6
Calibration curve range	2.05 - 500 pg/mL
Analysis sample	Mouse Serum/Plasma (EDTA) /Culture medium (D-MEM etc.)*
Sample volume	50 μL (2x dilution)
Measurement duration	Approx. 3 hours and 50 minutes
Wavelength	Primary wavelength : 450 nm Reference wavelength : 620 nm

• It is tested by using the samples that standard substances have been added to the culture medium. Measurement availability depends on the culture medium.
  When using cell culture supernatant as a sample, please make sure to perform recovery test and dilution linearity test on the culture medium before use.

Example of Calibration Curve

Measurement of IL-6 in Mouse Serum

[Result]
This product demonstrated high detection sensitivity, successfully quantifying IL-6 within the quantification range in 4 out of 5 samples.

1. Ono, Y. and Kotani, J.: Endotoxin and Innate Immunity, 25, 37(2024). (Japanese)
   Role of IL-6/JAK2/STAT3 signaling in skeletal muscle atrophy
2. Ogata, A.: Clin Rheumatol，27, 228(2015). (Japanese)
   IL-6 targeting in rheumatoid arthritis
3. Hirano, T. et al.: immunity., 52(5), 731(2020).
   COVID-19: A New Virus, but a Familiar Receptor and Cytokine Release Syndrome