Chromogranin A ELISA Kits

Chromogranin A (CgA) is an acidic glycoprotein that coexists and is co-released with catecholamine in the adrenal medulla and sympathetic nerve endings. CgA is thought to reflect secretion of catecholamine and is used as an activity index for the sympathetic-adrenal-medullary (SAM) axis, a stress-response pathway. It is also used as a tumor marker for patients with pheochromocytomas or neuroendocrine tumors, who have high levels of CgA in the blood.

CgA is also present in saliva, is synthesized in the submandibular gland, and is released into saliva in response to autonomic stimulation. Previous studies have reported that saliva CgA levels increase in response to stress^{1) 2)}, and thus, it is particularly useful as an excellent noninvasive biomarker for mental stress.

Human Chromogranin A ELISA Kit Wako is an ELISA kit for assaying CgA in human saliva and plasma.

Kit Performance

The range of the calibration curve	0.14 - 33.33 pmol/mL
Assay target	Chromogranin A
Sample	Human Saliva/Plasma
The sample amount required	50 µL （n=2）
Assay time	O/N+2.5 hours
Detection method	Colorimetry
Within assay variation	CV＜ 14％
Between assay variation	CV＜ 16％

Example of Calibration Curve

Performance Data

Spiked recovery test

Sample	Amount spiked (pmol/mL)	Measured value (pmol/mL)	Recovery rate (%)
Plasma	0	0.54	－
	0.25	0.86	108.86
	1	1.93	125.32
	4	6.58	144.93
Saliva	0	0.47	－
	0.25	0.81	112.50
	1	1.40	95.24
	4	3.66	81.88

1) Nakane, H. et al.: Biomed. Res., 19(6), 401(1998).

Salivary chromogranin A as an index of psychosomatic stress response

2) Takatsuji, K. et al.: Biomed. Res., 29(4), 221(2008).

The effects of examination stress on salivary cortisol, immunoglobulin A, and chromogranin A in nursing students