Silica Gel Packing Agent for HPLC Columns (Unmodified, C18 Modified)
Silica gel has a strong physical strength and is the most widely used as a column packing agent for analysis and purification in chromatographies such as HPLC, medium and low pressure chromatography, and open column chromatography.
In general, there are unmodified silica gel and chemically modified silica gel that has been changed their functions by binding of various functional groups (C18, NH2 etc.) to the silanol groups on the surface of silica gel.
FUJIFILM Wako provides lineups of spherical silica gel (Wakosil® 5SIL series) that is used as a packing agent for HPLC columns used for normal-phase chromatography and spherical silica gel (Wakosil® C18 series) that is used as a packing agent for HPLC columns used for reverse-phase chromatography.
List of Physical Properties for each Silica Gel Grade
| Silica gel for HPLC columns used for normal-phase chromatography (unmodified) | ||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features |
| Wakosil® 5SIL series | ||||||||
| Wakosil® 5SIL | Spherical | 4.0～6.0 μm | 6 nm | - | 500 m²/g | 1.0～4.0 | Silicagel for HPLC columns used for normal-phase chromatography | Silica gel for HPLC columns used for normal-phase chromatography. Product name: The number after 5SIL- = pore size. Shape: Totally porous spherical silica gel Use: HPLC column packing agent used for normal-phase chromatography |
| Wakosil® 5SIL-120 | 12 nm | 300 m²/g | ||||||
| Silica gel for HPLC columns used for reverse-phase chromatography（C18） | ||||||||||||||||
| Product Name | Shape | Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use | Features | ||||||||
| Wakosil® C18 series | ||||||||||||||||
| Wakosil® 5C18 | Spherical | 5 μm | 15 nm | 1.2 mL/g | 300 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography | This is the most common C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Product name: The number before C18 = particle size, -200 = Pore size 200Å (20 nm) Shape: Spherical silica gel modified with C18 group. (ODS) Use: HPLC columns packing agent for reverse-phase chromatography |
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| Wakosil® 10C18-200 | Spherical | 10 μm | 20 nm | 1 mL/g | 200 m²/g | |||||||||||
| Wakosil® C18 RS series | ||||||||||||||||
| Wakosil®-II 3C18 RS | Spherical | 3.0～3.7 μm | 9.5～11.5 nm | 0.95～1.10 mL/g | 390～430 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography | This series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Because its specific surface area is larger than that of Wakosil® 5C18, this series has a high retention function and separability of highly polar compounds. Product name: The number before C18 = particle size. Shape: Spherical silica gel modified with C18 group (ODS). Use: HPLC columns packing agent for reverse-phase chromatography |
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| Wakosil®-II 5C18 RS | Spherical | 4.2～4.7 μm | 11～13 nm | 1.15～1.30 mL/g | 390～430 m²/g | |||||||||||
| Wakosil® C18 HG | ||||||||||||||||
| Wakosil®-II 3C18 HG | Spherical | 3.2～3.7 μm | 11～13 nm | 0.85～1.00 mL/g | 280～320 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography | This series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. This silica gel has the highest number of theoretical plates of the C18 series and is suitable for separating basic compounds and metal coordination compounds. Product name: The number before C18 = particle size. Shape: Spherical silica gel modified with C18 group (ODS) Use: HPLC columns packing agent for reverse-phase chromatography |
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Wakosil® 5SIL Series
Wakosil® 5SIL series is totally porous spherical silica gel that is used as a packing agent for HPLC columns used for normal-phase adsorption chromatography.
Physical Property Information
| Product Name | Shape | Particle Size | Pore Size | Specific Area | pH | Intended Use |
|---|---|---|---|---|---|---|
| Wakosil®5SIL | Spherical | 4.0～6.0 μm | 6 nm | 500 m2/g | 1.0～4.0 | Silica gel for HPLC columns used for normal-phase chromatography |
| Wakosil®5SIL-120 | 12 nm | 300 m2/g |
Wakosil® C18 Series
Wakosil® C18 series is the most common C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography.
Physical Property Information
| Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
|---|---|---|---|---|---|---|---|
| Wakosil®5C18 | Spherical | 5 μm | 15 nm | 1.2 mL/g | 300 m2/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography |
| Wakosil®10C18-200 | 10 μm | 20 nm | 1 mL/g | 200 m2/g |
Wakosil® C18 RS Series
Wakosil® C18 RS Series is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. Because its specific surface area is larger than that of Wakosil® 5C18, this series has a high retention function and separability of highly polar compounds.
Physical Property Information
| Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
|---|---|---|---|---|---|---|---|
| Wakosil®-II 3C18 RS | Spherical | 3.0～3.7 μm | 9.5～11.5 nm | 0.95～1.10 mL/g | 390～430 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography |
| Wakosil®-II 5C18 RS | 4.2～4.7 μm | 11～13 nm | 1.15～1.30 mL/g |
Wakosil® C18 HG
Wakosil® C18 HG is the C18 groups-modified silica gel (ODS) for HPLC columns used for reverse-phase chromatography. This silica gel has the highest number of theoretical plates of the C18 series and is suitable for separating basic compounds and metal coordination compounds.
Physical Property Information
| Product Name | Shape | Particle Size | Pore Size | Pore Capacity | Specific Area | pH | Intended Use |
|---|---|---|---|---|---|---|---|
| Wakosil®-II 3C18 HG | Spherical | 3.2～3.7 μm | 11～13 nm | 0.85～1.00 mL/g | 280～320 m²/g | 2.0～7.5 | Silica gel for HPLC columns used for reverse-phase chromatography |
Product List
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Wakosil® 5SIL Series
Wakosil® C18 Series
Wakosil® C18 RS Series
Wakosil® C18 HG
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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