[NIPPON GENE] Buffers for Molecular Biology
NIPPON GENE's buffers are strictly quality controlled to ensure safe use for molecular biology.
Buffer quality control
A. DNase test
Added 18 μL of buffer to 1 μg of λ/Hind III and pBR322 DNA, incubated at 37°C for 16 hours, and electrophoresed on agarose gel. The pattern was shown not to have changed.
B. RNase test
Added 18 µL of buffer to 2 µg of E. coli 16S and 23S rRNA, incubated at 37°C for 16 hours, and electrophoresed on agarose gel. The pattern was shown not to have changed.
C. Blotting/hybridization test
It has been confirmed that general blotting and hybridization can be performed without interference.
D. Electrophoresis test
It has been confirmed that general electrophoresis can be performed without interference.
E. Ethanol precipitation test
It has been confirmed that general ethanol precipitation can be performed without interference.
Test items to be performed
| Product Name | Tests |
|---|---|
| 10M Ammonium Acetate | A, B, E |
| DEPC treated Water | A, B |
| Distilled Water, Deionized, Sterile | A, B |
| 0.5M EDTA (pH8.0) | A, B |
| Loading Buffer | D |
| 1M MgCl2 | A, B |
| 6 x Loading Buffer Double Dye | D |
| 6 x Loading Buffer Triple Dye | D |
| 6 x Loading Buffer Orange G | D |
| 10 x PBS Buffer (pH 7.4) | A, B |
| SDS-PAGE 10 x Running Buffer | D* |
| 3M Sodium Acetate | A, B, E |
| 20 x SSC | A, B, C |
| 10 x TAE, 50 x TAE | D |
| 5 x TBE | D |
| 10 x TBS Buffer (pH 7.4) | A, B |
| 20 x TBS (pH 7.4) | A, B |
| TE (pH 8.0) | A, B |
| 1M Tris-HCl (各pH) | A, B |
*It has been confirmed that SDS-PAGE can be performed without interference.
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Stock Solutions
Phenols
Buffers for Electrophoresis
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.