Disposable Homogenizer - BioMasher Series
BioMasher: Disposable homogenizer
BioMasher is used to disrupt the tissue sample when extracting RNA, DNA, and proteins from the sample.
The BioMasher lineup includes the BioMasher I, II, III, V, and SP models.
Comparison of RNA extraction efficiency among BioMasher I, II, and III
RNA was extracted from various organs of a mouse (Crlj:CD1 [ICR]: Adult mouse of 8 weeks or older) using BioMasher I-III according to the attached protocol. As a control, RNA was extracted by using a pestle of SSI and a 1.5 mL tube of TreffLab.
The results are summarized below.
Tissue RNA Extraction Ratio (RNA (μg)/Sample (mg)) BioMasher I BioMasher II BioMasher III Pestle/SSI & 1.5 mL tube/TreffLab Liver 1 1.5 1.32 0.92 Kidney 0.26 1.87 4.66 0.15 Cerebrum 0.16 1.14 - 0.12 Spleen 0.15 5.53 - 0.36 Heart 0.08 1.02 0.5 0.12 Tail 0.03 0.43 - 0.16 Muscle 0.02 0.8 0.24 0.43
*Choose appropriate model of BioMasher for the maximum extraction ratio of RNA depending on tissue type.
BioMasher I consists of a filter tube and a pestle. The sample is placed in the filter tube and the pestle is inserted from above. Centrifugal force is applied with a centrifuge allowing the pestle to crush the sample with this force. This allows the sample to pass through the filter of the filter tube, enabling the sample to be disrupted. The fibrous membrane component of the sample will be caught by the filter.
The pestle has a cross-shaped blade at the tip. By pushing and turning the pestle manually, the sample, which is hard to disrupt by centrifugation alone, can be ground.
With an O-ring
BioMasher I with an O-ring is suitable for homogenizing soft tissue with centrifugal force alone. In particular, it is suitable for simultaneous processing of many samples and for infectious samples.
Without an O-ring
Hard tissue may not be homogenized sufficiently by centrifugal force alone. The model without an O-ring is suitable when grinding the sample on the filter area before centrifugation to enhance disruption efficiency.
|* The material of the filter is available made of either polyethylene (PE) or polypropylene (PP).
The PE filter has smaller pore size than the PP filter resulting in finer disruption with the PE filter.
* DO NOT add the buffer in the filter tube before centrifuge. Otherwise the filter may be clogged, and the tube may be damaged. For only samples with lots of fibrous components with little water content, such as epidermis, mesentery, tail etc., add a small amount of buffer to extract and filtrate the target molecules.
BioMasher II consists of a 1.5 mL microtube and a pestle. The sample and extraction buffer are placed in the tube, and ground to prepare the homogenate.
The microtube and pestle are molded so that they fit without any space between them. The texture of the inner wall of the tube and the outer periphery of the tip of the pestle enhances the efficiency of the disruption of the sample.
The pestle has a splash guard, which is designed to fit the tube without unnecessary spaces. BioMasher II can be used to disrupt various samples, such as animal tissue/organ, plants (stems, roots, seeds), and insects.
|* For samples that are difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.
* DO NOT autoclave BioMasher II. In the case sterilization is required, please use a sterilized product.
Advantages of BioMasher II
BioMasher III has the features of both BioMasher I and II. This model consists of a filter tube with an abrasive surface inner wall and a pestle with a textured surface.
After grinding the sample in the filter tube using the pestle, centrifuge the extraction liquid. The extracted sample will be filtered and caught in the tube.
Since the fibrous membrane component get trapped by the filter, BioMasher III is recommended for homogenization of highly fibrous samples.
|* For samples that are difficult to homogenize, an exclusive electric stirrer (Nippi PowerMasher II) can be used for ease.
* BioMasher III CAN be autoclave in the case sterilization is required.
BioMasher V is a larger volume version of BioMasher III. After homogenizing the sample with a combination of the tube with a textured inner wall and the stirring pestle with an abrasive surface, centrifuge and filtrate with the filter.
Recommended for homogenizing highly fibrous samples.
|* For samples difficult to homogenize, an exclusive electric stirrer (PowerMasher II) is available for ease.
* DO NOT autoclave BioMasher V. In the case sterilization is required, please use a sterilized product.
BioMasher SP is a larger volume version of BioMasher II. This model has enhanced disruption efficacy from the combination of the 15 mL tube with an abrasive inner wall and a stirring pestle with a textured tip.
The shovel-like shape cutout and the helical groove of the pestle effectively trap and homogenize the tissue.
BioMasher SP is suitable for disruption of animal tissue/organ, plants (stems, roots, seeds), and insects.
|* For samples difficult to disrupt, an exclusive electric stirrer (Nippi PowerMasher II) is available for easy disruption.
* DO NOT autoclave BioMasher SP. In the case sterilization is required, please use a sterilized product.
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