Anti-glioma Antibodies for Immunohistochemistry

Glioma is a tumor formed by neuroglial cells, which support neurons.
The 2016 revision of the WHO criteria includes molecular information-based classification criteria in addition to conventional morphological diagnostic criteria. Researches have called attention to IDH (Isocitrate Dehydrogenase) 1/2 genes, ATRX gene, mutation in TERT promoter, and 1p/19q co-deletion as factors for molecular diagnosis of glioma.
We provide a wide range of monoclonal antibodies appropriate for immunohistochemical staining/western blotting to recognize wild-type/mutant IDH1, IDH2, ATRX, and TERT.

Anti-ATRX, Monoclonal Antibody (AMab-6)

ATRX (α-thalassemia/mental retardation syndrome X-linked) is a protein involved in transcriptional regulation and chromatin remodeling. ATR-X syndrome has been known to be caused by mutation in ATRX gene on the X chromosome.
This product is a monoclonal antibody that recognizes human ATRX protein. It can be used to detect ATRX mutation, which leads to loss of ATRX protein expressionn, by immunohistochemical staining.

Application Data

Immunohistochemical staining of oligodendroglioma [A] and diffuse astrocytoma [B]

01223_img01.gif
Sample: Paraffin-embedded tissue section of a lesion from a patient with brain tumor
A:Oligodendroglioma, IDH-mutant [WHO grade Ⅱ]
B:Diffuse astrocytoma, IDH-mutant [WHO grade Ⅱ]
  Section Thickness: 4 μm
  Antigen Activation Conditions: Solution at pH 9.0 was autoclaved for 10 minutes followed by a 40-minute storage at room temperature.
  Quenching: 3% Hydrogen peroxide solution, 10 minutes
  Blocking: 5% Skim milk solution, 30 minutes



Primary Antibody: 3 μg/mL Anti-ATRX monoclonal antibody (AMab-6)
Secondary Antibody: HRP-conjugated anti-mouse IgG (commercial ly-available product)
 Detection: DAB
 Counterstaining: Hematoxylin


A:Oligodendroglioma, IDH-mutant :
  No mutation in ATRX gene. pPositive immunohistochemical staining with anti-ATRX antibody.
B:Diffuse astrocytoma, IDH-mutant :
  Mutation in ATRX gene present, and loss of ATRX expression. Negative immunohistochemical staining with anti-ATRX antibody.
  Nucleus of vascular endothelial cells (normal cells) was positively stained with anti-ATRX antibody.





Data by courtesy of Dr. Yukinari Kato at Tohoku University Graduate School of Medicine.

References

  1. Ogasawara, S., Fujii, Y., Kaneko, M. K., Oki, H., Sabit, H., Nakada, M., Suzuki, H., Ichimura, K., Komori, T. and Kato, Y.: “Establishment of anti-Human ATRX monoclonal antibody AMab-6.”, Monoclonal Antibodies in Immunodiagnosis and Immunotherapy, 35(5), 254-258 (2016).

Product Overview

・Clone No. AMab-6
・Specificity: ATRX
・Host: Mouse
・Isotype: IgG1・κ
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (f1 μg/mL~)
           Immunohistochemistry (1~5 μg/mL)
           ELISA (1 μg/mL~)

※Please consider optimum concentrations depending on your experimental system./p>

Anti-TERT, Monoclonal Antibody (TMab-6)

Telomerase is a complex consisting of TERT (Telomerase Reverse Transcriptase), template RNAs, and regulatory subunits. TERT is the catalytic subunit of telomerase. Telomerase activity has been known to be retained not only in germ cells and tissue stem cells but also in 80%-90% of tumor cells at a high level.
This product is a monoclonal antibody against TERT, the catalytic subunit of telomerase, and is especially useful for immunohistochemical staining. The expression level of TERT protein has been known to be modified in some subtypes of glioma due to mutation in TERT promoter region. This phenomenon and aberration in ATRX gene are mutually exclusive. Mutation in TERT promoter is observed in almost all cases of glioma with IDH mutation and 1p/19q co-deletion, and also in glioma types with no IDH mutation.

Product Overview

・Clone No. TMab-6
・Specificity: TERT
・Host: Mouse
・Isotype: IgM・κ
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (1 μg/mL~)
           Immunohistochemistry (1 μg/mL~)
           ELISA (1 μg/mL~)

※Please consider optimum concentrations depending on your experimental system.

Anti-IDH, Monoclonal Antibodies

IDH (Isocitrate Dehydrogenase) is an oxidoreductase that interconverts isocitric acid and α-ketoglutarate. In humans, there are 3 types: IDH1 (cytosolic form, NADP+ dependent), IDH2 (mitochondrial form, NADP+ dependent), and IDH3 (mitochondrial form, NAD+ dependent).
IDH1IDH1 and IDH2 genes have been reported to be frequently mutated in various cancers including bile duct carcinoma, acute myeloid leukemia, chondrosarcoma, osteosarcoma, and glioma.
We provide a wide range of antibody products that recognize IDH1, IDH2, and wild-type/mutant IDH1/2.

Anti-IDH1-R132H, Monoclonal Antibody (HMab-1) [Cat. No. 018-24081]

It has been reported that mutation in IDH1 is almost limited to Arg at position 132, and R132H mutation, in which Arg at position 132 is converted to His, accounts for 80%-90%.
This product is an antibody that specifically recognizes IDH1-R132H, in which Arg at position 132 is mutated to His. It recognizes neither wild-type IDH1 nor IDH1 with non-R132H mutation.

Product Overview

・Clone No. HMab-1
・Specificity: IDH1-R132H
・Host: Mouse
・Isotype: IgG1
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (5 μg/mL)
           Immunohistochemistry (5~10 μg/mL)
           ELISA (1~5 μg/mL)

※Please consider optimum concentrations depending on your experimental system.

References

  1. Takano, S. et al.J. Neurooncol., 108(3), 361-373 (2012).
  2. Sabit, H. et al.Brain Tumor Pathol., 31(4), 242-246 (2014).
  3. Takano, S. et al.Brain Tumor Pathol., 32(3), 169-175 (2015).
  4. Kato, Y.: Brain Tumor Pathol., 32(1), 3-11 (2015).

Anti-IDH1-R132H, Monoclonal Antibody (HMab-2) [Cat. No. 013-26851]

This product, like Clone No. HMab-1, is an antibody that specifically recognizes IDH1-R132H. It recognizes neither wild-type IDH1 nor IDH1 with non-R132H mutation.
It provides a high sensitivity even at low antigen or antibody levels.

Product Overview

・Clone No. HMab-2
・Specificity: IDH1-R132H
・Host: Mouse
・Isotype: IgG1・κ
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (1 μg/mL~)
           Immunohistochemistry (1 μg/mL)
           ELISA (1 μg/mL~)

※Please consider optimum concentrations depending on your experimental system.

References

  1. 1.Fujii, Y. et al.Biochem. Biophys. Res. Commun., 466(4), 733-739 (2015).
  2. 2.Yamamichi, A. et al.Sci. Technol. Adv. Mater., 17, 618-625 (2016).

Anti-IDH1-R132S, Monoclonal Antibody (SMab-1) [Cat. No. 015-24091]

There are IDH1 mutations in which Arg at position 132 is converted to Cys, Ser, Gly, or Leu rather than to His.
This product is a monoclonal antibody that recognizes IDH1-R132S, in which Arg at position 132 is converted to Ser.

Product Overview

・Clone No. SMab-1
・Specificity: IDH1-R132S
・Host: Mouse
・Isotype: IgG1
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (5 μg/mL)
           Immunohistochemistry (5~10 μg/mL)
           ELISA (1~5 μg/mL)

※Please consider optimum concentrations depending on your experimental system.

References

  1. Kaneko, M. K. et al.Biochem. Biophys. Res. Commun., 406(4), 608-613 (2011).
  2. Kato, Y.: Brain Tumor Pathol., 32(1), 3-11 (2015).

Anti-IDH1 monoclonal antibody (RMab-3) [Cat. No. 014-24061]

This product is an antibody that recognizes wild-type/mutant IDH1. It can be used for immunohistochemical staining of tissue sections from various types of glioma.

Product Overview

・Clone No. RMab-3
・Specificity: Wild type and mutated IDH1
・Host: Mouse
・Isotype: IgG1
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (5 μg/mL)
           Immunohistochemistry (5~10 μg/mL)
           ELISA (1~5 μg/mL)

※Please consider optimum concentrations depending on your experimental system.

References

  1. Kaneko, M. K., et al.Tohoku J. Exp. Med., 230, 103-109 (2013).
  2. Ogasawara, S. et al.Monoclon. Antib. Immunodiagn. Immunother., 35(5), 254-258 (2016).

Anti-IDH1, Rat Monoclonal Antibody (RcMab-1) [Cat. No. 014-26381]

This product is a monoclonal antibody that recognizes wild-type/mutant IDH1. It does not recognize IDH2.
It can be used for immunohistochemical staining of brain tissue sections and western blotting of brain-derived samples as positive or loading control antibody.

Product Overview

・Clone No. RcMab-1
・Specificity: Wild type and mutated IDH1
・Host: Rat
・Isotype: IgG2a
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (0.5 to 1 μg/mL)
           Immunohistochemistry (1~5 μg/mL)
           Immunocytochemistry(1~5 μg/mL)

※Please consider optimum concentrations depending on your experimental system./p>

References

  1. Ikota, H. et al.Brain Tumor Pathol., 32(4), 237-244 (2015).
  2. Kaneko, M.K. et al.Tohoku J. Exp. Med., 230, 103-109 (2013).
  3. Kato, Y. et al.Biochem. Biophys. Res. Commun., 432, 546-567 (2013).
  4. Kaneko, M.K. et al.Monoclon. Antib. Immunodiagn. Immunother., 32(3), 224-228 (2013).
  5. Yamamichi, A. et al.Sci. Technol. Adv. Mater., 17(1), 618-625 (2016).

Anti-IDH2, Monoclonal Antibody (RMab-22) [Cat. No. 011-24071]

This product is a monoclonal antibody that recognizes wild-type/mutant IDH2.
In mutant IDH2, Arg at position 172 is converted to Lys, Met, Gly, or Trp.
This antibody recognizes both wild-type IDH2 and IDH2-R172K/M mutant. It does not recognize IDH2-R172W.

Product Overview

・Clone No. RMab-22
・Specificity: Wild type IDH2 and mutated IDH2 (IDH2-R172K/R172M)
・Host: Mouse
・Isotype: IgG2b
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (5 μg/mL)
           Immunohistochemistry (5~10 μg/mL)
           ELISA(1~5 μg/mL)

※Please consider optimum concentrations depending on your experimental system.

References

  1. 1.Kaneko, M.K.et al.Biochem. Biophys. Res. Commun., 432, 40-45 (2013).

Anti-mutant IDH1/2, Monoclonal Antibody (MsMab-1) [Cat. No. 015-25691]

This product is an antibody that recognizes mutated IDH1 and mutated IDH2. It does not recognize wild-type IDH1/2.
Specifically, it recognizes IDH1 mutants IDH1-R132H/R132S/R132G and IDH2 mutants IDH2-R172M/R172S/R172G.

Product Overview

・Clone No. MsMab-1
・Specificity: Mutated IDH1 (IDH1-R132H/R132S/R132G) and mutated IDH2 (IDH2-R172M/R172S/R172G)
・Host: Mouse
・Isotype: IgG2a・κ
・Formulation: Liquid/PBS containing sodium azide
・Antibody Concentration: Approximately 1 mg/mL (stated on the product label)
・Application: Western Blot (1 μg/mL)
           Immunohistochemistry (5 μg/mL)
           ELISA(1 μg/mL)
・Reactivity: IDH1: R132S, R132G > R132H (WB)
      IDH2 R172G > R172S > R172N (WB)

※Please consider optimum concentrations depending on your experimental system.

References

  1. Kaneko, M.K. et al.Tohoku J.Eep. Med., 230, 103-109 (2013).
  2. Liu, X. et al.Cancer Med., 2(6), 803-814 (2013).
  3. Ogasawara, S. et al.Monoclon. Antib. Immunodiagn. Immunother., 32(6), 377-381 (2013).
  4. Takano, S. et al.Brain Tumor Pathol., 32(3), 169-175 (2015).

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