EV-Save™ Extracellular Vesicle Blocking Reagent
- EV-Save™ strongly suppresses adsorption of extracellular vesicles in culture supernatant or after purification to laboratory tools
- You only need to add EV-Save™ to the sample
Precautions for use
- The effect of the product cannot be obtained when it is used for serum, plasma, or samples containing a lot of impurities.
- The product contains polymer. Do not use the product if the polymer may have effect on the subsequent processes of the experiment.
Adsorption of purified extracellular vesicles is suppressed
A sample containing purified extracellular vesicle 3 ng/μL was prepared from COLO201 cells, added to a tube, transferred to a new tube, and allow to stand still for 3 minutes. Reduction in extracellular vesicles was determined by PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before transfer (100%).
·Loss by adsorption at transfer is almost completely suppressed
·Stability during storage is improved
Decrease in extracellular vesicles during ultrafiltration is prevented
Reduction in extracellular vesicles after ultrafiltration for concentration (Vivaspin20, 100K dalton) of human iPScell culture supernatant was evaluated using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before ultrafiltration (100%).
Extracellular vesicles are not lost during ultrafiltration
Recovery of purified extracellular vesicles is improved
Extracellular vesicles were purified from 1 mL of TIG3 cell culture supernatant using MagCapture™ Exosome Isolation Kit PS, and extracellular vesicles were measured using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained from input (100%).
Recovery by MagCapture™ Exosome Isolation Kit PS is improved
Use in cell experiments
To TBS solution containing human iPScells, EV-Save™ was added at a ratio of 1/100, and the resultant TBS solution was added to culture medium at a ratio of 1/5. Human iPScells were cultured on this medium for 3 days, and viable cell count was determined. Graph shows CD63 signal relative to that obtained from a sample without EV-Save™(100%).
No effect on proliferation or pluripotency of human iPS cells
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