To suppress adsorption of extracellular vesicles to laboratory tools

EV-Save™ Extracellular Vesicle Blocking Reagent

Features

  • EV-Save™ strongly suppresses adsorption of extracellular vesicles in culture supernatant or after purification to laboratory tools
  • You only need to add EV-Save™ to the sample

Precautions

Precautions for use

  • The effect of the product cannot be obtained when it is used for serum, plasma, or samples containing a lot of impurities.
  • The product contains polymer. Do not use the product if the polymer may have effect on the subsequent processes of the experiment.

Data 1

Adsorption of purified extracellular vesicles is suppressed

01116_img01.png

A sample containing purified extracellular vesicle 3 ng/μL was prepared from COLO201 cells, added to a tube, transferred to a new tube, and allow to stand still for 3 minutes. Reduction in extracellular vesicles was determined by PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before transfer (100%).



·Loss by adsorption at transfer is almost completely suppressed
·Stability during storage is improved

Data 2

Decrease in extracellular vesicles during ultrafiltration is prevented

01116_img02.png

Reduction in extracellular vesicles after ultrafiltration for concentration (Vivaspin20, 100K dalton) of human iPScell culture supernatant was evaluated using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before ultrafiltration (100%).





Extracellular vesicles are not lost during ultrafiltration

Data 3

Recovery of purified extracellular vesicles is improved

01116_img03.png

Extracellular vesicles were purified from 1 mL of TIG3 cell culture supernatant using MagCapture™ Exosome Isolation Kit PS, and extracellular vesicles were measured using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained from input (100%).



Recovery by MagCapture™ Exosome Isolation Kit PS is improved

Data 4

Use in cell experiments

01116_img04.png

To TBS solution containing human iPScells, EV-Save™ was added at a ratio of 1/100, and the resultant TBS solution was added to culture medium at a ratio of 1/5. Human iPScells were cultured on this medium for 3 days, and viable cell count was determined. Graph shows CD63 signal relative to that obtained from a sample without EV-Save™(100%).



No effect on proliferation or pluripotency of human iPS cells

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Product content may differ from the actual image due to minor specification changes etc.

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