EV-Save™ Extracellular Vesicle Blocking Reagent
Features
- EV-Save™ strongly suppresses adsorption of extracellular vesicles in culture supernatant or after purification to laboratory tools
- You only need to add EV-Save™ to the sample
Precautions
Precautions for use
- The effect of the product cannot be obtained when it is used for serum, plasma, or samples containing a lot of impurities.
- The product contains polymer. Do not use the product if the polymer may have effect on the subsequent processes of the experiment.
Data 1
Adsorption of purified extracellular vesicles is suppressed
A sample containing purified extracellular vesicle 3 ng/μL was prepared from COLO201 cells, added to a tube, transferred to a new tube, and allow to stand still for 3 minutes. Reduction in extracellular vesicles was determined by PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before transfer (100%).
·Loss by adsorption at transfer is almost completely suppressed
·Stability during storage is improved
Data 2
Decrease in extracellular vesicles during ultrafiltration is prevented
Reduction in extracellular vesicles after ultrafiltration for concentration (Vivaspin20, 100K dalton) of human iPScell culture supernatant was evaluated using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained before ultrafiltration (100%).
Extracellular vesicles are not lost during ultrafiltration
Data 3
Recovery of purified extracellular vesicles is improved
Extracellular vesicles were purified from 1 mL of TIG3 cell culture supernatant using MagCapture™ Exosome Isolation Kit PS, and extracellular vesicles were measured using PS Capture™ Exosome ELISA Kit (Anti Mouse IgG POD). Graph shows CD63 signal relative to that obtained from input (100%).
Recovery by MagCapture™ Exosome Isolation Kit PS is improved
Data 4
Use in cell experiments
To TBS solution containing human iPScells, EV-Save™ was added at a ratio of 1/100, and the resultant TBS solution was added to culture medium at a ratio of 1/5. Human iPScells were cultured on this medium for 3 days, and viable cell count was determined. Graph shows CD63 signal relative to that obtained from a sample without EV-Save™(100%).
No effect on proliferation or pluripotency of human iPS cells
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