It has been added to the cart.

It has been added to the comparison table.

EV-Save™ Extracellular Vesicle Blocking Reagent

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
058-09261
Barcode No
4548995068828
1mL
List Price
108.00 USD

In stock

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Procedure

For isolation of extracellular vesicles from cell culture supernatants using Mag Capture™ Exosome Isolation Kit PS (Code No. 299-77603, 293-77601)

  1. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into cell culture supernatants and mix it by inverting or tapping.
    In the case of concentrating cell culture supernatants by an ultrafiltration unit, add EV-Save™ Extracellular Vesicle Blocking Reagent into cell culture supernatants before ultrafiltration.
  2. Proceed with an experiment to the step of “Elution of extracellular vesicles” described in the instruction of MagCapture™ Exosome Isolation Kit PS.
  3. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into “Exosome Elution Buffer” accompanied with Mag Capture™ Exosome Isolation Kit PS and mix it by inverting or tapping.
  4. Elute extracellular vesicles according to the instruction of Mag Capture™ Exosome Isolation Kit PS.
  5. Store the eluted extracellular vesicles, or proceed to the next experiment.

For isolation of extracellular vesicles from cell culture supernatants using other methods

  1. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100- fold into cell culture supernatants and mix it by inverting or tapping.
  2. Add EV-Save™ Extracellular Vesicle Blocking Reagent to be diluted 100-fold into isolated extracellular vesicles and mix it by inverting or tapping.
  3. Store it or proceed to the next experiment

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
It is a polymeric reagent that suppresses adsorption of extracellular vesicles such as Exosome in cell culture supernatant to laboratory instruments/containers.
Since extracellular vesicles adsorb to sample tubes and ultrafiltration membranes, they are significantly reduced during purification and storage. This product strongly suppresses adsorption of such extracellular vesicles. When MagCapture™ Exosome Isolation Kit PS (Code No. 299-77603, 293-77601)is used to purify extracellular vesicles from cell culture supernatant, use of this product is strongly recommended.
[Before use]
·If you use this product for serum, plasma or contaminated samples, the effect of this product will not be obtained.
·This product contains polymer. Do not use this product if there is a risk that the existence of polymer will affect the results of the experiment in the subsequent process.

Property

Appearance Colorless - nearly colorless, clear liquid
Density 1.005 - 1.011g/mL (20 degrees C)

Manufacturer Information

Alias

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

877-714-1920

Hours of Operation: 8:00 - 17:00 (EST)For other hours than the above, please contact us via the inquiry form.