Ordering
NH2 Silica Gel 60 F254 Plate-Wako

Amino Silica Gel TLC plate

Amino (NH2) Silica Gel 60 F254 Plate Wako is a thin layer chromatography (TLC) plate coated with aminopropyl group modified silica gel. This TLC plate can be used in normal phase, reversed phase, ion exchange mode based on the characteristics of the adsorbent. It is particularly suitable for separation of basic compounds. This product contains an indicator emitting green fluorescence with ultraviolet light (λ=254 nm). Therefore, substances that absorb ultraviolet light (λ=254 nm) can be observed dark spots on a green background.
FUJIFILM Wako has TLC plates with layer thicknesses of 0.25 mm, 0.5 mm and 0.75 mm. The layer thickness of 0.25 mm is suitable for inspection and research, and there are two plate sizes: 20 cm × 20 cm and 6.6 cm × 2.5 cm. Layer thicknesses of 0.5 mm and 0.75 mm are suitable for preparative purification.

Application

Separation for nucleotides

Sample: each 10mg/mL (2mL)
Mobile phase: acetonitrile/methanol/water=80/10/10(v/v/v)

Thickness; 0.25 mm Thickness; 0.5 mm
sample development Rf value development Rf value
thymidine 40mm 0.36 40mm 0.38
adenosine 31mm 0.28 31mm 0.27
cytidine 15mm 0.13 14mm 0.12
(front) 112mm - 113mm -

Separation for dyes

Sample: each 10mg/mL (2mL)
Mobile phase: LiCl/methanol=1/100(w/v)
Thickness: 0.25 nm

sample amount = 1 mL sample amount = 2 mL
sample development Rf value development Rf value
acid red 52 68mm 0.61 68mm 0.61
brilliant blue FCF 60mm 0.54 60mm 0.54
eosin Y 46mm 0.41 47mm 0.42
new coccine 24mm 0.22 24mm 0.23
(front) 111mm - 111mm -

Separation for amines

TLC Conditions
TLC ①Silica Gel 70F254 Plate-Wako
②NH2 Silica Gel 60F254 Plate-Wako
Sample 1) 2,4,6-Trimethylpyridine (left spot)
2) 4-Dimethylaminopyridine (right spot)
Mobile phase: n-Hexane/IPA = 90/10 (v/v)

Product List

  • Open All
  • Close All

Related Product List

  • Open All
  • Close All

Product content may differ from the actual image due to minor specification changes etc.

Author: Y. Suto

877-714-1920

Hours of Operation: 8:00 - 17:00 (EST)For other hours than the above, please contact us via the inquiry form.