Anti asialo GM1 (Rabbit)/Antibody for Cancer Research

We have a product line of various antibodies for cancer research, including Anti asialo GM1 (Rabbit).

Anti asialo GM1, rabbit

The glycolipid asialo ganglioside-GM1 (ASGM1) is expressed by T cells in viral infection and by natural killer (NK) cells. ASGM1 is localized in lipid raft structures in NK and CD8(+) T cells. Anti asialo GM1 antibody reduces natural killer (NK) activity in cells of various strains of mice and rats.
To investigate the function of NK cells, or transplant tumor tissue derived from the other species to mice, Anti asialo GM1 antibody is injected to remove NK cells1)2).
Recently, it is reported that ASGM1 is also expressed by Basophils, and Anti asialo GM1 antibody is able to remove Basophils too3).

● Contents Rabbit anti asialo GM1, lyophilized
● Preparation GM1 from bovine brain tissue was repeatedly immunized with methylated bovine serum albumin and with complete Freund’s adjuvant.
● Purification Gammaglobulin fraction of serum was obtained by 50% ammonium sulfate precipitation methods followed by dialyzing with phosphate buffered saline (pH7.2).
● Specificity React with mouse and rat natural killer cells, mouse monocytes and fetal thymocytes.
● Reconstitution Reconstitute with 1 mL of distilled water. For dilution, phosphate buffer (pH7.2) is recommended.
●Storage Aliquotting into one by one is recommended. Store at freezer. Avoid freeze thaw cycles.
● Injection Inject to mouse 10-50μL intravenously.

Features

  • Tested the anti NK cells activityin vivo
  • Used in many papers

References

  1. Kasai M., Iwamori M., Nagai Y., Okumura K., and Tada T. : J. Immunol., 10, 175 (1980).
  2. Habu S., Fukui H., Shimamura M., Kasai M., Nagai Y., Okumura K., and Tamaoki N. : J. Immunol., 127, 34 (1981).
  3. Hideto, N., Kaori, M., Yohei, K., Yoshiyuki, M., and Hajime K., .: J. Immunol., 186, 10, 5766(2011).

Anti asialo GM1, rabbit: References

Our Anti asialo GM1 used in many papers as follows;

  • Hudson, W. A., Li, Q., Le, C., and Kersey, J. H., : Leukemia, 12, 2029 (1998).
  • Murphy W. J., Durum S. K., Longo D. L., : PNAS, 89, 10, 4481 (1992).
  • Bentzmann S., Roger P., Dupuit F., Bajolet-Laudinat O., Fuchey C., Plotkowski C. M., Puchelle E. : Infection and immunity, 64, 5, 1582 (1996).
  • Nishikado H., Mukai K., Kawano Y., Minegishi Y., Karasuyama H. : J. Immunol., 186, 10, 5766(2011).
  • Victorino, F., Sojka, K. D., Brodsky, S. K. McNamee, N. E., Masterson, C. J., Homann, D., Yokoyama, M. W., Eltzschig, K. H., Clambey, T. E., : J. Immunol., 195, 10, 4973(2015).

Titration of Anti asialo GM1 in vitro

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Spleen cells of BALB/c※ were treated with Anti Asialo GM1 plus guinea pig complement. Remaining NK activities were tested in vitro by using YAC-1 cells as target(●). Effector/target ratio was 50:1. ○ represents NK activities of BALB/c spleen cells treated with complement.


※BALB/c was injected with 100 μg of polyinosinic-polycytidylic acid sodium salt (0.2mL of 500μg/mL Poly I:C) and maintained for 18 hours before next procedure.

Titration of Anti asialo GM1 in vivo

Anti asialo GM1 was intraperitoneally injected to BALB/c mice. Pancreatic cells isolated 3 days after the one-shot injection were used as effector cells for measurement of tumor lysis against YAC-1 cells (effector/target = 50:1).


※ BALB/c mice were injected with 100 μg of polyinosinic-polycytidylic acid sodium salt (0.2 mL of 500 μg/mL Poly I:C) and maintained for 18 hours before the next procedure.

Control: normal rabbit serum
Volume injected intraperitoneally Tumor lysis (%)
100 μL 70.8
Anti asialo GM1 antibody
Volume injected intraperitoneally Tumor lysis (%)
10 μL 7.0
25 μL 8.4
50 μL 2.0
100 μL 0.0

List of antibodies for cancer research

Target Product Code Product Name Clone No. Cross-reactivity Application Package
Size
Host Isotype
Asialo GM1 986-10001 Anti asialo GM1,Monoclonal Antibody Poly Mouse, Rat Neu 1 mL
Rabbit IgG, IgA, IgM
ATRX 017-26751 Anti ATRX, Monoclonal Antibody AMab-6 Human IHC, WB, ELISA 100 µg
Mouse IgG1・κ
HB-EGF 013-27191 Anti HB-EGF, Monoclonal Antibody Y-073 Human, Mouse, Rat Neu, ELISA 10 µL
019-27193 Mouse IgG1・κ 50 µL
Mutated/Normal IDH1 014-26381 Anti IDH1, Rat Monoclonal Antibody RcMab-1 Human, Hamster ICC, IHC, WB 100 µg
Rat IgG2a
IDH1-R132H mutation 018-24081 Anti IDH1-R132H, Monoclonal Antibody HMab-1 IHC, WB, ELISA 100 µg
Mouse IgG1
IDH1-R132H mutation 013-26851 Anti IDH1-R132H, Monoclonal Antibody HMab-2 Human IHC, WB, ELISA 100 µg
Mouse IgG1・κ
IDH1-R132S mutation 015-24091 Anti IDH1-R132S, Monoclonal Antibody SMab-1 IHC, WB, ELISA 100 µg
Mouse IgG1
Mutated/Normal IDH2 011-24071 Anti IDH2, Monoclonal Antibody RMab-22 Human, Mouse, Hamster IHC, WB, ELISA 100 µg
Mouse IgG2b
Mutated IDH1/2 015-25691 Anti Mutated IDH1/2, Monoclonal Antibody MsMab-1 IHC, WB, ELISA 100 µg
Mouse IgG2a・κ
Podocalyxin 011-27491 Anti Human Podocalyxin, Monoclonal Antibody PcMab-47b Human FC, IHC, ELISA 100 µg
Mouse IgG1・κ
Podoplanin 018-24101 Anti Human Podoplanin, Monoclonal Antibody NZ-1.2 Human FC, IHC, IP, WB, ELISA 100 µg
Rat IgG2a
Podoplanin 013-27071 Anti Human Podoplanin, Monoclonal Antibody LpMab-10 Human FC, ICC, IHC, WB, ELISA 100 µg
Mouse IgG3・κ
Podoplanin 014-27001 Anti Human Podoplanin, Monoclonal Antibody LpMab-12 Human FC, IHC, WB, Neu, ELISA, 100 µg
Mouse IgG1・κ
Podoplanin 011-27011 Anti Human Podoplanin, Monoclonal Antibody LpMab-13 Human FC, IHC, WB, ELISA 100 µg
Mouse IgG1・κ
Podoplanin 018-27021 Anti Human Podoplanin, Monoclonal Antibody LpMab-17 Human FC, IHC, WB, ELISA 100 µg
Mouse IgG1・κ
Podoplanin 017-27471 Anti Human Podoplanin, Monoclonal Antibody LpMab-19 Human FC, IHC, ELISA 100 µg
Mouse IgG2b・κ
Podoplanin 014-27481 Anti Human Podoplanin, Monoclonal Antibody LpMab-21 Human FC, IHC, ADCC,CDC,ELISA 100 µg
Mouse IgG2a・κ
Podoplanin 015-27031 Anti Rat Podoplanin, Monoclonal Antibody PMab-2 Rat FC, IHC, WB,ELISA 100 µg
Mouse IgG1・κ
Podoplanin 012-27041 Anti Rabbit Podoplanin, Monoclonal Antibody PMab-32 Rabbit FC, IHC, WB,ELISA 100 µg
Mouse IgG1・κ
Podoplanin 015-24111 Anti Mouse Podoplanin, Monoclonal Antibody PMab-1 Mouse FC, IHC,IP, WB,ELISA 100 µg
Mouse IgG2a
Podoplanin 017-27091 Anti Dog Podoplanin, Monoclonal Antibody PMab-38 Dog FC, IHC,WB,ELISA 100 µg
Mouse IgG2a
TERT 010-26861 Anti TERT, Monoclonal Antibody TMab-6 Human HC,WB,ELISA 100 µg
Mouse IgM・κ
List of abbreviations representing applications
FC : Flow cytometry ICC : Immunocytochemical staining IHC : Immunohistochemical staining IHC(F) : Immunohistochemical staining (frozen sections) IHC(P) : Immunohistochemical staining (paraffin section
IP : Immunoprecipitation WB : Western blotting Neu : Exhibits neutralizing activity ADCC : Exhibits antibody-dependent cellular cytotoxicity CDC : Complement-dependent cytotoxici

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