Organic Phosphorus Pesticide Mixture Standard Solutions
Organophosphorus pesticides are widely used mainly as insecticides. Organophosphorus pesticides inhibit the action of cholinesterase and makes it difficult to decompose acetylcholine, which is a neurotransmitter, which causes impaired neurotransmission. Since organophosphorus pesticides are highly toxic to the human, their use is being reduced worldwide.
FUJIFILM Wako provide 4 types of organic phosphorus pesticide mixed standard solutions as FA series. 56 compounds of organophosphorus pesticides can be analyzed simultaneously.
FA-1
GC Chromatogram
| Peak No. | Constituent | Peak No. | Constituent | Peak No. | Constituent |
|---|---|---|---|---|---|
| 1 | Dichlorvos (DDVP) | 9 | Chlorpyrifosmethyl | 17 | Fosthiazate |
| 2 | Methamidophos | 10 | Pirimiphos-methyl | 18 | Propaphos |
| 3 | Acephate | 11 | (E)-Dimethylvinphos | 19 | Profenofos |
| 4 | Cadusafos | 12 | Malathion (Malathon) | 20 | Ethion |
| 5 | Salithion | 13 | (Z)-Dimethylvinphos | 21 | Edifenphos (EDDP) |
| 6 | Diazinon | 14 | (E)-Chlorfenvinphos (α-CVP) | 22 | Cyanofenphos (CYP) |
| 7 | Iprobenfos (IBP) | 15 | Quinalphos | 23 | Pyridaphenthion |
| 8 | Cyanophos (CYAP) | 16 | (Z)-Chlorfenvinphos (β-CVP) | 24 | Phosalone |
Conditions
[ Instrument ]
Shimadzu GC-2014
[GC]
Column:DB-1701 0.25 μm, 0.25 mm × 30 m
Column temperature: 60 ℃ (2 min)→25 ℃/min→150 ℃→5 ℃/min→270 ℃ (20 min)
Injection temperature: 250 ℃
Carrier gas: He 1.0 mL/min
Split ratio: 1/10
Detector temperature: 270 ℃
*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixed standard solution.
FA-2
GC Chromatogram
| Peak No. | Constituent | Peak No. | Constituent | Peak No. | Constituent |
|---|---|---|---|---|---|
| 1 | Ethoprophos | 9 | Chlorpyrifos | 17 | Butamifos |
| 2 | Phorate | 10 | Formothion※ | 18 | Sulprofos |
| 3 | Thiometon | 11 | Fenthion (MPP) | 19 | Fensulfothion |
| 4 | Terbufos | 12 | Fenitrothion (MEP) | 20 | EPN |
| 5 | Etrimfos | 13 | Isofenphos | 21 | Phosmet (PMP) |
| 6 | Dichlofenthion (ECP) | 14 | Phenthoate (PAP) | 22 | Pyraclofos |
| 7 | Dimethoate※ | 15 | Prothiofos | ||
| 8 | Tolclofos-methyl | 16 | Methidathion (DMTP) |
*Organophosphorus pesticides may be adsorbed/decomposed in the injection port or column.
For example, when analyzed by splitless injection, formothion may partially decompose to dimethoate.
Conditions
[ Instrument ]
Shimadzu GC-2014
[GC]
Column: DB-1701 0.25 μm, 0.25 mm × 30 m
Column temperature: 60 ℃ (2 min)→25 ℃/min→150 ℃→5 ℃/min→270 ℃ (20 min)
Injection temperature: 250 ℃
Carrier gas: He 1.0 mL/min
Split ratio: 1/10
Detector temperature: 270 ℃
*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixed standard solution.
FA-3
GC Chromatogram
| Peak No. | Constituent | Peak No. | Constituent | Peak No. | Constituent |
|---|---|---|---|---|---|
| 1 | Omethoate | 5 | Fenamiphos | 9 | Azinphos-ethyl |
| 2 | Disulfoton (Ethylthiomethon) | 6 | Isoxathion | 10 | Coumaphos |
| 3 | Monocrotophos | 7 | Vamidothion | ||
| 4 | Bromophos-ethyl | 8 | Azinphos-methyl |
Conditions
[ Instrument ]
Shimadzu GC-2014
[GC]
Column: DB-1701 0.25 μm, 0.25 mm × 30 m
Column temperature: 60 ℃ (2 min)→25 ℃/min→150 ℃→5 ℃/min→270 ℃ (25 min)
Injection temperature: 250 ℃
Carrier gas: He 1.0 mL/min
Split ratio: 1/10
Detector temperature: 270 ℃
*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixed standard solution.
Organophosphorus Pesticides Mixture Standard Solution
GC Chromatogram
| Peak No. | Constituent | Peak No. | Constituent |
|---|---|---|---|
| 1 | Methyl Demeton | 3 | Parathion |
| 2 | Methylparathion | 4 | EPN |
Conditions
[ Instrument ]
Shimadzu GC-2014
[GC]
Column: BP1 (SGE) 1.0 μm, 0.53 mm × 25 m
Column temperature: 100 ℃ (1 min)→20 ℃/min→260 ℃ (11 min)
Injection temperature: 150 ℃
Carrier gas: He 6.0 mL/min
Split ratio: 1/5
Detector temperature: 280 ℃
*Analysis conditions, charts, and detected ions are reference data. It is not the data to guarantee the pesticide mixed standard solution.
Data
LC/MS/MS analysis example using Wakopak®
In LC/MS/MS analysis using FA series Wakopak®, 50 (52 components) of the 54 items (56 components) were confirmed.
-
HPLC Conditions Columun: Wakopak Wakosil-Ⅱ 3C18 HG 2.0×150 mm(W)、C/N20029(9873) Eluent: A)0.005% acetic acid and 5 mM ammonium acetate in H2O B)CH3CN Gradient: 0-6 min. B=5-40 % 6-22 min. B=40-90 % 22-27 min. B=90 % 27-37 min. B=5 % Frow rate: 0.2 mL/min at 40 ℃ Injevtion vol: 3 μL、0.1 ppm -
MS/MS Conditions IonSpray Voltage(V) 4500 Temperature(℃) 350 Curtain GAS 20 Collision GAS 5 Ion Source Gas1 70 Ion Source Gas2 60
- The chromatogram shows the groups with similar sensitivity.
- The displayed chromatogram is due to "Product 1" of the product ion in the list.
- These data are examples of analysis, not for product quality assurance.
| No. | Product Name | RT | Precursor | Product1 | Product2 | |
|---|---|---|---|---|---|---|
| 1 | Dimethoate | 8.6 | 230.0 | 125.0 | 199.0 | FA-2 |
| 2 | Fosthiazate | 12.1 | 284.0 | 104.0 | 228.0 | FA-1 |
| 3 | Pyridaphenthion | 15.8 | 341.0 | 189.0 | 205.0 | FA-1 |
| 4 | lprobenfos | 16.7 | 289.1 | 91.1 | 205.1 | FA-1 |
| 5 | Propaphos | 17.2 | 305.0 | 221.0 | 141.0 | FA-1 |
| 6 | Edifenphos | 17.6 | 311.0 | 109.1 | 111.1 | FA-1 |
| 7 | Etrimfos | 18.8 | 293.1 | 125.0 | 265.0 | FA-2 |
| 8 | Cadusafos | 19.0 | 271.0 | 159.0 | 131.0 | FA-1 |
| 9 | Diazinon | 19.2 | 305.0 | 169.0 | 153.0 | FA-1 |
| 10 | Pirimiphos-methyl | 20.2 | 306.1 | 108.0 | 164.1 | FA-1 |
| 11 | Fensulfothion | 12.8 | 309.1 | 281.1 | 157.0 | FA-2 |
| 12 | Dimethylvinphos | 15.3(Z)、16.0(E) | 331.0 | 127.0 | 170.0 | FA-1 |
| 13 | Etoprophos | 15.9 | 243.1 | 131.0 | 97.0 | FA-2 |
| 14 | Malathion | 16.6 | 331.0 | 99.0 | 285.0 | FA-1 |
| 15 | Chlorfenvinphos | 17.7(α)、18.4(β) | 359.0 | 155.0 | 99.0 | FA-1 |
| 16 | Methamidophos | 3.2 | 142.0 | 94.0 | 125.0 | FA-1 |
| 17 | Acephate | 4.5 | 184.0 | 143.1 | 95.0 | FA-1 |
| 18 | Omethoate | 5.1 | 214.0 | 182.9 | 124.9 | FA-3 |
| 19 | Vamidothion | 7.7 | 288.0 | 146.0 | 118.0 | FA-3 |
| 20 | Methidathion | 14.6 | 303.0 | 145.0 | 85.0 | FA-2 |
| 21 | Phosmet | 15.0 | 318.0 | 160.0 | 133.0 | FA-2 |
| 22 | Quinalphos | 18.1 | 299.1 | 97.1 | 243.1 | FA-1 |
| 23 | Pyraclofos | 18.5 | 361.1 | 257.1 | 138.1 | FA-2 |
| 24 | Phenthoate | 18.7 | 321.0 | 163.0 | 79.0 | FA-2 |
| 25 | Phosalone | 19.7 | 368.0 | 182.0 | 111.0 | FA-1 |
| 26 | Butamifos | 20.1 | 333.1 | 96.0 | 180.1 | FA-2 |
| 27 | Profenofos | 20.3 | 373.0 | 303.0 | 345.0 | FA-1 |
| 28 | lsofenphos | 20.3 | 346.1 | 245.0 | 217.0 | FA-2 |
| 29 | Ethion | 22.4 | 385.1 | 199.1 | 171.1 | FA-1 |
| 30 | Monocrotophos | 6.6 | 224.0 | 98.0 | 127.0 | FA-3 |
| 31 | Dichlorvos | 10.7 | 221.0 | 109.0 | 127.0 | FA-1 |
| 32 | Azinphos-methyl | 14.6 | 318.0 | 132.0 | 124.9 | FA-3 |
| 33 | Fenamiphos | 15.2 | 304.0 | 217.0 | 202.0 | FA-3 |
| 34 | Azinphos-ethyl | 17.2 | 346.0 | 160.1 | 132.1 | FA-3 |
| 35 | Phorate | 19.7 | 261.0 | 75.0 | 199.0 | FA-2 |
| 36 | lsoxathion | 19.9 | 314.0 | 105.0 | 170.0 | FA-3 |
| 37 | Terbufos | 21.9 | 289.0 | 103.0 | 57.0 | FA-2 |
| 38 | Chlorpyrifos | 22.3 | 350.0 | 198.0 | 97.0 | FA-2 |
| 39 | Sulprofos | 22.7 | 323.0 | 219.0 | 139.0 | FA-2 |
| 40 | Formothion | 11.4 | 258.0 | 199.0 | 125.0 | FA-2 |
| 41 | Salithion | 14.7 | 217.0 | 107.0 | 123.1 | FA-1 |
| 42 | Cyanophos | 15.9 | 244.0 | 125.0 | 134.0 | FA-1 |
| 43 | Fenthion | 18.6 | 279.0 | 169.0 | 105.0 | FA-2 |
| 44 | Coumaphos | 19.1 | 363.0 | 307.0 | 226.9 | FA-3 |
| 45 | Cyanofenphos | 19.2 | 304.0 | 157.0 | 276.0 | FA-1 |
| 46 | Tolclofos-methyl | 19.7 | 301.0 | 125.0 | 175.0 | FA-2 |
| 47 | Disulfoton | 20.0 | 275.1 | 89.0 | - | FA-3 |
| 48 | EPN | 20.2 | 324.0 | 157.0 | 296.0 | FA-2 |
| 49 | Bromophos-ethyl | 24.3 | 394.8 | 366.9 | 338.9 | FA-3 |
| 50 | Prothiofos | 24.8 | 345.0 | 241.0 | 133.0 | FA-2 |
Components not detected: Chlorpyriphos-methyl (FA-1) 、Dichlofenthion (FA-2) 、Thiometon (FA-2) 、Fenitrothion (FA-2)
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Mixed standard solution
Organophosphorus pesticide standard
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.