近赤外/赤外色素

Jackson社 Alexa Fluor® 680、Alexa Fluor® 790標識体

近赤外/赤外色素は、可視光色素に比べ、蛍光消光が少ない、吸光係数が大きい、および、自家蛍光によるバックグラウンドが低いため、より高感度な検出が可能です。近赤外/赤外色素の使用で蛍光強度が上がることにより、多様な免疫蛍光検出、画像診断や、各種ウェスタンブロット (quantitative Western blots、in-gel Westerns、microWestern arrays、in-cell Western arrays、on-cell Western arrays)、組織切片イメージング等多くの手法が利用できます。

Jackson社は、Alexa Fluor® 680、Alexa Fluor® 790の標識⼆次抗体、ストレプトアビジン、免疫グロブリン等をラインアップしています。

  • BSAやスキムミルクはウシIgGを含有している可能性があり、ここでご紹介している標識二次抗体と反応する恐れがあります。ブロッキング剤や希釈液にBSAやスキムミルクを使用すると、バックグラウンドを高め、抗体価を下げることがありますのでご注意ください。

蛍光スペクトル

  • 励起波長 (nm) 蛍光波長 (nm)
    Alexa Fluor® 680 684 702
    Alexa Fluor® 790 792 803

Alexa Fluor® 680 (近赤外色素) とAlexa Fluor® 790 (赤外色素) を用いたウェスタンブロットにおける免疫二重染色例

β-メルカプトエタノールとSDSで還元、変性させたマウスIgGとヤギIgG (バックグラウンドコントロール) 各2 μgを、SDS-PAGEで電気泳動し、軽鎖と重鎖に分離後、ニトロセルロース膜に転写した。
1:100,000希釈したAlexa Fluor® 790 AffiniPure Goat Anti-Mouse IgG, Fcγ subclass 1 specific (min X Hu, Bov, Rb Sr Prot) (メーカーコード:115-655-205) で重鎖を検出 (緑) 。
1:100,000希釈したAlexa Fluor® 680 AffiniPure Goat Anti-Mouse IgG, light chain specific (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig) (メーカーコード︓115-625-174) で軽鎖を検出 (赤) 。
LiCor Odyssey imager使用。

上記二種類の抗体は、 1:100,000希釈でありながら、非常に高い蛍光強度で検出した。

標識体

Anti-Chicken (ニワトリ)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Chicken IgY (IgG) (H+L)
(min X Bov, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)
703-625-155 703-655-155
Product Information Product Information
Fab Fragment Goat Anti-Chicken IgY (IgG), Fc fragment specific 103-627-008 103-657-008
Product Information Product Information

Anti-Goat (ヤギ)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Fab Fragment Bovine Anti-Goat IgG, Fc fragment specific 805-627-008 805-657-008
Product Information Product Information
Donkey Anti-Goat IgG (H+L)
(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)
705-625-147 705-655-147
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Goat IgG, light chain specific
(min X Hrs, Hu, Ms, Rb, Rat Ig)
205-622-176 205-652-176
Product Information Product Information

Anti-Guinea Pig (モルモット)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Guinea Pig IgG (H+L)
(min X Bov, Ck, Gt, Sy Hms, Hrs, Hu, Ms, Rb, Rat, Shp Sr Prot)
706-625-148 706-655-148
Product Information Product Information
Fab Fragment Goat Anti-Guinea Pig IgG, Fc fragment specific 106-627-008 106-657-008
Product Information Product Information

Anti-Armenian Hamster (アルメニアンハムスター)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Goat Anti-Armenian Hamster IgG (H+L)
(min X Bov, Hu, Ms, Rb, Rat Sr Prot)
127-625-160 127-655-160
Product Information Product Information

Anti-Human (ヒト)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Human IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Ms, Rb, Rat, Shp Sr Prot)
709-625-149 709-655-149
Product Information Product Information
Fab Fragment Goat Anti-Human IgG, Fcγ fragment specific 109-627-008 109-657-008
Product Information Product Information
Goat Anti-Human IgG, Fcγ fragment specific
(min X Bov, Hrs, Ms Sr Prot)
109-625-098 109-655-098
Product Information Product Information
Goat Anti-Human IgG, Fcγ Fragment Specific
(min X Bov,Ms Sr Prot)
109-625-190 109-655-190
Product Information Product Information
Goat Anti-Human IgG, Fcγ fragment specific
(min X Bov, Ms, Rb Sr Prot)
109-625-170 109-655-170
Product Information Product Information
Fab Fragment Goat Anti-Human IgM, Fc fragment specific 109-627-043 109-657-043
Product Information Product Information
Goat Anti-Human IgM, Fc fragment specific
(min X Bov Sr Prot)
109-625-129 109-655-129
Product Information Product Information
Goat Anti-Human Serum IgA, α Chain Specific 109-625-011 109-655-011
Product Information Product Information

Anti-Mouse (マウス)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Mouse IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot)
715-625-151 715-655-151
Product Information Product Information
Donkey Anti-Mouse IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)
715-625-150 715-655-150
Product Information Product Information
Goat Anti-Mouse IgG (H+L)
(min X Hu, Bov, Hrs, Rb, Rat Sr Prot)
115-625-166 115-655-166
Product Information Product Information
Goat Anti-Mouse IgG (H+L)
(min X Hu, Bov, Hrs, Rb, Sw Sr Prot)
115-625-146 115-655-146
Product Information Product Information
Goat Anti-Mouse IgG, light chain specific
(min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig)
115-625-174 115-655-174
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ fragment specific
(min X Hu, Bov, Hrs Sr Prot)
115-625-071 115-655-071
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgG1, Fcγ fragment specific 115-627-185 115-657-185
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ subclass 1 specific
(min X Hu, Bov, Rb Sr Prot)
115-625-205 115-655-205
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgG2a, Fcγ fragment specific 115-627-186 115-657-186
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ subclass 2a specific
(min X Hu, Bov, Rb Sr Prot)
115-625-206 115-655-206
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgG2b, Fcγ fragment specific 115-627-187 115-657-187
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ subclass 2b specific
(min X Hu, Bov, Rb Sr Prot)
115-625-207 115-655-207
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgG2c, Fcγ fragment specific 115-627-188 115-657-188
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgG3, Fcγ fragment specific 115-627-189 115-657-189
Product Information Product Information
Goat Anti-Mouse IgG, Fcγ subclass 3 specific
(min X Hu, Bov, Rb Sr Prot)
115-625-209 115-655-209
Product Information Product Information
Fab Fragment Goat Anti-Mouse IgM, µ chain specific 115-627-020 115-657-020
Product Information Product Information
Goat Anti-Mouse IgM, µ chain specific
(min X Hu, Bov, Hrs Sr Prot)
115-625-075 115-655-075
Product Information Product Information

Anti-Rabbit (ウサギ)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Rabbit IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot)
711-625-152 711-655-152
Product Information Product Information
Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) 111-625-144 111-655-144
Product Information Product Information
Fab Fragment Goat Anti-Rabbit IgG, Fc fragment specific 111-627-008 111-657-008
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, light chain specific
(min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig)
211-622-171 211-652-171
Product Information Product Information

Anti-Rat (ラット)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Rat IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot)
712-625-153 712-655-153
Product Information Product Information
Donkey Anti-Rat IgG (H+L)
(min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot)
712-625-150 712-655-150
Product Information Product Information
Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Rb Sr Prot) 112-625-167 112-655-167
Product Information Product Information
Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot) 112-625-143 112-655-143
Product Information Product Information
Goat Anti-Rat IgG, light chain specific
(min X Bov, Gt, Hrs, Hu, Ms, Rb, Shp Ig)
112-625-175 112-655-175
Product Information Product Information
Fab Fragment Goat Anti-Rat IgG, Fcγ fragment specific 112-627-008 112-657-008
Product Information Product Information
Goat Anti-Rat IgG, Fcγ fragment specific
(min X Hu, Bov, Hrs Sr Prot)
112-625-071 112-655-071
Product Information Product Information
Fab Fragment Goat Anti-Rat IgM, µ chain specific 112-627-020 112-657-020
Product Information Product Information
Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) 112-625-075 112-655-075
Product Information Product Information

Anti-Sheep (ヒツジ)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey Anti-Sheep IgG (H+L)
(min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot)
713-625-147 713-655-147
Product Information Product Information
IgG Fraction Monoclonal Mouse Anti-Sheep IgG, light chain specific
(min X Bov, Hrs, Hu, Ms, Rb, Rat Ig)
213-622-177 213-652-177
Product Information Product Information

Anti-Biotin (ビオチン)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
IgG Fraction Monoclonal Mouse Anti-Biotin 200-622-211 200-652-211
Product Information Product Information

Anti-Digoxin (ジゴキシン)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
IgG Fraction Monoclonal Mouse Anti-Digoxin 200-622-156 200-652-156
Product Information Product Information

Anti-Fluorescein (フルオレセイン、FITC)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
IgG Fraction Monoclonal Mouse Anti-Fluorescein (FITC) 200-622-037 200-652-037
Product Information Product Information

Anti-His Tag (Hisタグ)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Rabbit Anti-His Tag 300-625-240 300-655-240
Product Information Product Information

Anti-Horseradish Peroxidase (ペルオキシダーゼ、HRP)

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Goat Anti-Horseradish Peroxidase 123-625-021 123-655-021
Product Information Product Information

Streptavidin

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Streptavidin 016-620-084 016-650-084
Product Information Product Information

IgG

抗体名 Alexa Fluor® 680
A=684,E=702
Alexa Fluor® 790
A=792,E=803
Donkey IgG, whole molecule 017-620-003 017-650-003
Product Information Product Information
Goat IgG, whole molecule 005-620-003 005-650-003
Product Information Product Information
Mouse IgG, whole molecule 015-620-003 015-650-003
Product Information Product Information
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