Microglia Remover
- for Cellbiology
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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- Packing
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1mL
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In stock in Japan |
Document
Product Overview
Microglia Remover is anionic liposomes suspension containing clodronic acid. When this product is phagocytosed by microglia as foreign substances, clodronic acid is released within the cells, causing cell death. By encapsulating clodronic acid in liposomes, it is taken up into microglia more efficiently than clodronic acid alone. Microglia remover (for Control) (sold separately, Product Number 131-19511) is also available as a control for experiments.
Features
- Microglia can be removed in approximately 1-3 days after administration
- Less expensive than commercial microglia removal feeds
Product Information
| Lipid composition | Dipalmitoylphosphatidylcholine, Dipalmitoylphosphatidylserine and Cholesterol |
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| Average particle size | 100 nm |
| Formulation | Phosphate Buffered Saline sterilized with 0.22 μm filter |
Protocol
Intracerebral infusion
- 1-2 μL Microglia Remover or Microglia Remover (for Control) are infused into the cerebral parenchyma.
- Assess the depletion of microglia in 1-3 days post-infusion.
Data
Application Data
Microglia removal in mouse cerebral cortex
After inserting a 33 G needle into the mouse cerebral cortex, 1 µL of Microglia Remover (for Control) or Microglia Remover (this product) was injected, respectively, and sampled after 3 days.
- Animal
- Mouse (C57BL6/J)
- Site
- Cerebral cortex
- Sample
- Frozen section
Antibody Concentration
- Primary Antibody
- Anti Iba1, Rabbit (for immunocytochemistry) (Product Number 019-19741) 1:500
- Secondary Antibody
- Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Product Number 111-545-144) 1:500
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
- [Result]
- In the control group, microglia (or macrophages) accumulated in large numbers at the site of injury.
On the other hand, the “Microglia Remover” group showed inhibition of the accumulation of microglia in the injured area and observed a decrease or regression in the number of microglia in the surrounding area.
FAQ
About usage
- Are there any precautions when using the product?
- Clodronate liposomes may precipitate after long-term storage. slowly invert the vial several times until the suspension appears homogeneous by visual inspection.
- Are there any precautions for storage?
- Avoid freezing and close the cap of the tube to prevent an influence on liposomes caused by oxidation.
About application
- Is it possible to remove macrophages?
- It is known that clodronate liposomes can also remove macrophages, which may be possible in principle, but we have not conducted any experiments.
Overview / Applications
| Outline | Microglia Remover is anionic liposomes suspension containing clodronic acid. When this product is phagocytosed by microglia as foreign substances, clodronic acid is released within the cells, causing cell death. By encapsulating clodronic acid in liposomes, it is taken up into microglia more efficiently than clodronic acid alone. Properties :Liquid Average particle size : 100 nm Buffer :PBS |
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| Application | 1. 1~2 uL Microglia Remover or Microglia Remover (for Control) are infused into the cerebral parenchyma. 2. Sampling is performed 1~3 days after injection and evaluated by immunohistochemistry or other methods. |
| Precautions for Use | 1. Avoid freezing. 2. If it is not used for a long period of time, slowly invert the vial several times until the suspension appears homogeneous by visual inspection. 3. When using Microglia Remover, take a sample from this product with sterilized instrument. 4. Close the cap of the tube to prevent an influence on liposomes caused by oxidation. |
Property
| Appearance | Liquid |
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Manufacturer Information
Alias
- Microglia Removal Reagent
For research use or further manufacturing use only. Not for use in diagnostic procedures.
Product content may differ from the actual image due to minor specification changes etc.
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