Human β Amyloid(1-42) ELISA Kit Wako, High Sensitive
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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96Tests
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In stock in Japan |
Document
Kit component
For 96 tests
| Antibody (BAN50)-coated Microplate (Anti Human Aβ [1-16] MoAb [Clone No. BAN50]) | 1 plate |
|---|---|
| Standard Solution (Human β amyloid [1-42], 20 pmol/L) | 2 mL x 2 |
| Standard Diluent | 30 mL x 1 |
| Wash Solution (20x) | 50 mL x 1 |
| HRP-conjugated Antibody (BC50) Solution (Snti Human Aβ [35-43] MoAb [Clone No. BC05] Fab’-HRP) | 12 mL x 1 |
| TMB Solution | 12 mL x 1 |
| Stop Solution | 12 mL x 1 |
| Plate Seal | 3 sheets |
Features
This ELISA kit enables highly sensitive quantification of β amyloid (1-42), which has been associated with Alzheimer’s disease.
The detection sensitivity of the kit is about 10 times higher than that of the conventional kit, with the standard curve range of 0.1-20.0 pmol/L. As with the conventional kit, non-specific binding is minimized by using Fab’ monoclonal antibody.
- Enables quantification of Aβ (1-42) with high sensitivity (standard curve range: 0.1-20.0 pmol/L).
- Has a low level of non-specific binding since Fab’ fragment of the antibody (BC05) is used to recognize the C-terminal.
- Consists of a highly specific monoclonal antibody developed by Takeda Pharmaceutical Company Ltd..
Performance
Standard curve
Standard curves were prepared using the standard solution included in the kit. The curves resembled a straight line between 0.1-20.0 pmol/L.
Spike-and-recovery test
Standard curves were prepared using the standard solution included in the kit. The curves resembled a straight line within the range between 0.1-20.0 pmol/L.
Dilution linearity test
Normal Human Plasma (A, C, E) were diluted 2-16 folds to determine the dilution linearity.
Specificity test
| Sample | Cross-reactivity (%) |
|---|---|
| Human Aβ(1-40) | ≦0.1 |
| Human Aβ(1-42) | 100.0 |
| Human Aβ(1-43) | 13.5 |
| Rat(Mouse) Aβ(1-40) | ≦0.1 |
| Rat(Mouse) Aβ(1-42) | 0.5 |
Application data
Measurements in human and mouse plasma
Blood samples collected in evacuated blood collection tubes with K2EDTA were centrifuged for 15 min. at 5,000 x g and 4°C. Plasma was isolated and kept in -80°C until use.
The plasma samples were diluted 4-fold using the standard diluent solution contained in the kit prior to the measurements.
(※Human β Amyloid(1-40) ELISA Kit Wako II (Cat. No. 298-64601) and Human/Rat β Amyloid(40) ELISA Kit Wako II (Cat. No. 294-64701) were used for the measurements of Aβ [1-40] and Aβ [x-40]).
(Data provided by: Drs. Iwatsubo and Hashimoto, Department of Neuropathology, Graduate School of Medicine, University of Tokyo)
Overview / Applications
| Outline | This product is for research use only. Do not administer it to human.
These ELISA kits detecting beta amyloid (1-42) and (x-42)* that ensure highly sensitive detection. These beta amyloids are indicated to have a correlation with Alzheimer's disease. Detection sensitivity of the kits is about 10 times higher than that of the existing productsand the dynamic range is from 0.1 to 20 pmol/L. Fab' fragment of the labeled antibody is used as well as so that the nonspecific binding is reduced. *: beta Amyloid (x-42) is beta Amyloid peptide modified or cleaved at the N-terminal.
[Kit Contents]
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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