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Anti Iba1, Monoclonal Antibody(NCNP24)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Cross‒Reactivity :
Mouse, Rat, Marmoset
Host :
Mouse
Clone No. :
NCNP24
Classification :
Monoclonal
Application :
IHC(Mouse, Rat and Marmoset frozen section, DAB)(1:500 - 2,000), IHC(Rat frozen section, fluorescent)(1:100)
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
012-26723
Barcode No
4548995057389
10uL
List Price
JPY 16,500

In stock in Japan

Distributor
016-26721
Barcode No
4548995056955
50uL
List Price
JPY 49,500

In stock in Japan

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Features

  • Tested and evaluated by immunohistochemistry
  • Mouse monoclonal antibody with high levels of staining and specificity

Application data

Immunohistochemistry

Rat Mouse Marmoset
Primary antibody
Anti-Iba1 monoclonal antibody (NCNP24)
Secondary antibody
Biotin-conjugated anti-mouse IgG antibody
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Primary antibody
Rabbit anti-Iba1 polyclonal antibody (019-19741)
Secondary antibody
Biotin-conjugated anti-rabbit IgG antibody
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Experimental conditions
Sample Frozen sections of the cerebral cortex from 7-week old Wistar rat, 7-week old ICR mouse, or adult marmoset
Staining method ABC method + DAB staining
Antibody concentration 1:500

The staining clearly revealed the structure of microglia. The staining was comparable to our rabbit anti-Iba1 polyclonal antibody (019-19741).

The data were provided by: Drs. Sanagi, Manabe, Ichinohe, and Kohsaka, National Center of Neurology and Psychiatry.

Overview / Applications

Outline Iba1 is a protein highly expressed in microglia and macrophage with a molecular weight of about 17 kDa. The protein is a commonly known as a microglial marker in the central nervous system.
Recently, microglia has been extensively studied because they are responsible for immune function in the central nervous system, and are related to various diseases such as neurodegenerative diseases, mental illuness, brain tumors and infection.

[Antigen]
C-terminal of Iba1

[Subclass]
Mouse IgG1

[Cross-reactivity]
Mouse, rat, marmoset

[Applications]
Immunohistochemistry (Frozen, DAB) 1:500-2,000

Property

Antibody Information

Antigen Iba1 (C-terminus)
Antigen Alias Iba1 (C-terminus)
Subclass IgG1,k
Labeling Unlabeled

Manufacturer Information

Alias

  • Iba1 Mouse monoclonal Antibody (Clone No.NCNP24)
    AIF1 Mouse monoclonal Antibody (Clone No.NCNP24)
    IRT1 Mouse monoclonal Antibody (Clone No.NCNP24)

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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