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Anti Iba1, Rabbit, SPICA Dye™ 594-conjugated

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
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List Price
JPY 55,000

In stock in Japan


Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate


Iba1 (Ionized calcium-binding adapter molecule1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system1). It is expressed in both resting and activated microglia but is reportedly expressed more highly in activated microglia2). It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis3).

Anti Iba1, Rabbit, SPICA Dye™ 594-conjugated is Anti Iba1, Rabbit (for Immunocytochemistry) (Product Number 019-19741) labeled with SPICA Dye™ 594, a proprietary fluorescent dye. It is possible to save time to detect with a secondary antibody.

Antibody Information

Clonality Polyclonal
Antigen Synthetic peptide (Iba1 C-terminal sequence)
Host Rabbit
Reactivity Mouse, Rat
Buffer PBS, 0.05% sodium azide
Conjugate SPICA Dye™ 594 (Ex=575 nm, Em=611 nm)
Applications Immunohistochemistry (Frozen Section) 1:200 - 2000

Application Data


  • Rat
  • Mouse

  • Species: Rat / Mouse
    Site: Cerebral cortex
    Sample: Frozen section
    Antibody concentration: 1:200


  1. Imai, Y., Ibata, I., Ito, D., Ohsawa, K., & Kohsaka, S.: Biochem.Biophys. Res. Commun., 224(3), 855(1996).
    A Novel Geneiba1 in the Major Histocompatibility Complex Class III Region Encoding an EF Hand Protein Expressed in a Monocytic Lineage
  2. Mori, I., Imai, Y., Kohsaka, S., & Kimura, Y.: Microbiol. Immunol., 44(8), 729(2000).
    Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection
  3. Sasaki, Y., Ohsawa, K., Kanazawa, H., Kohsaka, S., & Imai, Y. Biochem. Biophys. Res. Commun., 286(2), 292(2001).
    Iba1 is an actin-cross-linking protein in macrophages/microglia.

Overview / Applications


Appearance Liquid

Manufacturer Information


  • Anti Iba1 Polyclonal Antibody, Rabbit, SPICA Dye™ 594-conjugated

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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