NH2 Silica Gel 60 F254 Plate-Wako

Amino Silica Gel TLC plate

Amino (NH2) Silica Gel 60 F254 Plate Wako is a thin layer chromatography (TLC) plate coated with aminopropyl group modified silica gel. This TLC plate can be used in normal phase, reversed phase, ion exchange mode based on the characteristics of the adsorbent. It is particularly suitable for separation of basic compounds. This product contains an indicator emitting green fluorescence with ultraviolet light (λ=254 nm). Therefore, substances that absorb ultraviolet light (λ=254 nm) can be observed dark spots on a green background.
FUJIFILM Wako has TLC plates with layer thicknesses of 0.25 mm, 0.5 mm and 0.75 mm. The layer thickness of 0.25 mm is suitable for inspection and research, and there are two plate sizes: 20 cm × 20 cm and 6.6 cm × 2.5 cm. Layer thicknesses of 0.5 mm and 0.75 mm are suitable for preparative purification.

Application

Separation for nucleotides

Sample: each 10mg/mL (2mL)
Mobile phase: acetonitrile/methanol/water=80/10/10(v/v/v)

Thickness; 0.25 mm Thickness; 0.5 mm
sample development Rf value development Rf value
thymidine 40mm 0.36 40mm 0.38
adenosine 31mm 0.28 31mm 0.27
cytidine 15mm 0.13 14mm 0.12
(front) 112mm - 113mm -

Separation for dyes

Sample: each 10mg/mL (2mL)
Mobile phase: LiCl/methanol=1/100(w/v)
Thickness: 0.25 nm

sample amount = 1 mL sample amount = 2 mL
sample development Rf value development Rf value
acid red 52 68mm 0.61 68mm 0.61
brilliant blue FCF 60mm 0.54 60mm 0.54
eosin Y 46mm 0.41 47mm 0.42
new coccine 24mm 0.22 24mm 0.23
(front) 111mm - 111mm -

Separation for amines

TLC Conditions
TLC ①Silica Gel 70F254 Plate-Wako
②NH2 Silica Gel 60F254 Plate-Wako
Sample 1) 2,4,6-Trimethylpyridine (left spot)
2) 4-Dimethylaminopyridine (right spot)
Mobile phase: n-Hexane/IPA = 90/10 (v/v)

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Author: Y. Suto

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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