Silica gel plate-Wako

TLC Plates

Our silica gel TLC plates offer two pore sizes: 7 nm (70 series) and 4 nm (40 series). The 40 series TLC plate has larger surface area, thus, its Rf value is lower than that of the 70 series. This enables analyzing very polar organic solvents. We offer two kinds of fluorescence indicators.One is an indicator (F254 series) which emits green fluorescence by ultraviolet light (λ=254 nm). Another is an indicator (FM series) that emits bluish white or white with broad-spectrum ultraviolet light (λ = 250 to 400 nm). FM series detects the target compound as a colored spot. In addition, the F254 series has stronger fluorescence intensity than other manufacturers.
Silica gel 70F254 TLC Plate-Wako is coated with porous silica gel having uniform particles in pore diameter of 7 nm (70 Å) evenly on its glass plate. After adding green fluorescent substances (70F254 and 70FM), the separated spots are observed as dark spots in the green background by irradiation of ultraviolet (254 nm). Another size of pore diameter, 4 nm (40 Å) is also available as Silica gel 40F254 TLC Plate-Wako.

Silica gel 70F254 series


  • The pore size of silica gel ranges; 5-15 μm.
  • Increased spot convergence, improved separation performance.
  • A new fixing agent is used. Excellent resistance to peeling and stability by organic solvent and chemical materials.
  • Two preparative TLCs with different layer thicknesses are prepared.


TLC Conditions
Solvent: CHCl3/CH3OH/Butanol = 90/5/5 (v/v/v)

 ①Noscapine ②Caffeine ③7-(β-Hydroxypropyl) Theophylline
 ④Mixture of doping agent ⑤Dibucaine Hydrochloride
 ⑥Procaine Hydrochloride ⑦Brucine

UV: λ=250-400nm

Physical Property

Particle diameter of silica gel 5 - 15 μm
Pore diameter of silica gel 7 nm (70 Å)
Specific surface area of silica gel 450 m2/g
Pore volume of silica gel 0.8 mL/g
Thickness of silica gel layer 230 - 250 μm
Support Glass plate
Fluorescent indicator Monochromatic fluorescent substance [254nm]

Silica gel 40F254 series


  • Correrates with separation of Presep® HC-N series for flash chromatography and useful for setting preparative conditions.
  • Small Rf value compared with conventional silica gel plates, and the developing solvent is effective to separate hardly soluble compounds by increasing the ratio of highly polar solvents.

Example of Silica gel 70F254 TLC Plate-Wako


  • Silica gel 70F254 TLC Plate-Wako

  • Other vendor, “A”



  1. caffeine
  2. naproxen
  3. p-acetamidophenol
  4. p-hydroxybenzoic acid

CHCl3/Methanol (=v/v)
 a) 95/5, b) 90/10, c) 85/15, d) 80/20

254 nm

10 cm

Example of Silica gel 70F254 TLC Plate-Wako and Presep® HC-N

  • Comparison with another
    • 00973_img04.png
    • Conditions
      Plate: 2 cm x 10 cm (flow to 7 cm)
      Sample: DBP 0.01 g/mL
      DMP 0.01 g/mL
      in Hex/AcOEt = 85/15
      Loading: 0.5 µL

      DBP = Dibutyl Phthalate
      DMP = Dimethyl Phthalate

    Ratio of polar solvent can be increased, effective for separating poorly soluble compounds.

    ① another TLC plate 85/15 0.41 0.21
    ② 40F254 TLC plate 85/15 0.31 0.11
    ③ 40F254 TLC plate 80/20 0.42 0.19

  • Relations between Rf value and column volume

    According to results, it is possible to estimate the elution time of the Presep® silica gel (HC-N) column.

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Presep® HC-N series

Wakosil® HC-N

Author: Y. Suto

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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