Mature BDNF ELISA Kit Wako
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
- GHS :
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96Tests
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Kit component
96 tests
| Antibody-coated Plate | 1 Plate |
|---|---|
| Mature BDNF Standard | 100 μL |
| Buffer | 60 mL |
| Biotin-conjugated Antibody Solution | 100 μL |
| Peroxidase-conjugated Streptavidin Solution | 100 μL |
| TMB Solution | 6 mL |
| Stop Solution | 6 mL |
| Wash Solution (10×) | 100 mL |
| Plate Seal | 4 Sheets |
Features
- High sensitivity
- Detects mBDNF specifically
- Small sample volume
Performance
| Standard curve range | 4.1 - 1,000 pg/mL |
|---|---|
| Specificity | mBDNF* |
| Sample | Human serum and plasma** |
| Sample volume | Human serum：10 μL Human plasma: 5 μL |
| Assay time | 4 hours |
| Detection method | Colorimetric method |
* Approximately 10% reaction to proBDNF
** This antibody can also detect mouse and rat BDNF.
Assay principle
This kit is a sandwich ELISA using mBDNF monoclonal antibody and mBDNF polyclonal antibody. The kit has low cross-reactivity to proBDNF(approx. 10%) because the monoclonal antibody reacts with N-terminal cutting surface of mBDNF.
| Fujifilm Wako | Company A | Company B | Company C | |
|---|---|---|---|---|
| Cross-reactivity to proBDNF | Approx. 10％ | Approx. 10％ | Approx. 15％ | Approx. 50％ |
| Minimum concentration of assay range |
4.1 pg/mL | 62.5 pg/mL | 15.6 pg/mL | 15.0 pg/mL |
Data
Spiked recovery test
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Sample Spike mBDNF (pg/mL) Assay value (pg/mL) Recovery volume (pg/mL) Recovery (%) Human serum
①0 330 - - 100 423 93.0 93.0 250 581 251 100 500 817 487 97.4 Human serum
②0 56.6 - - 50 106 49.4 98.8 100 154 97.4 97.4 200 257 200 100 300 363 306 102 -
Sample Spike mBDNF (pg/mL) Assay value (pg/mL) Recovery volume (pg/mL) Recovery (%) Human plasma
①0 156 - - 100 252 96.0 96.0 250 407 251 100 500 661 505 101 Human plasma
②0 66.0 - - 50 115 49.0 98.0 100 164 98.0 98.0 200 276 210 105 300 369 303 101
Dilution linearity
Example of measurement -clinical sample -
Their serum mBDNF levels were measured by this kit. The samples were from healthy controls and patients with major depression, bipolar disorder, or schizophrenia.
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Healthy control (n=6)
No. Sex Age Assay value(ng/mL) 1 F 48 14.5 2 F 50 22.8 3 F 54 20 4 F 47 18.5 5 F 42 13.8 6 F 60 19.1 -
Major depression (n=6)
No. Sex Age Assay value (ng/mL) 1 F 66 15.7 2 F 38 18.1 3 F 43 10.0 4 F 52 16.6 5 F 41 22.3 6 F 58 15.1
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Bipolar disorder (n=4)
No. Sex Age Assay value (ng/mL) 1 F 46 16.5 2 F 52 20.9 3 F 52 11.5 4 F 52 11.5 -
Schizophrenia (n=4)
No. Sex Age Assay value(ng/mL) 1 F 36 17.9 2 F 53 23 3 F 55 14.6 4 F 51 10.8
| Healthy person | Major depression | Bipolar disorder | Schizophrenia |
| 18.1 ± 3.41 | 16.3 ± 4.02 | 15.1 ± 4.53 | 16.6 ± 5.17 |
(ng/mL)
Overview / Applications
| Outline | This item is designed for the quantitative detection and measurement of mature BDNF in biological fluids such as serum, and plasma. Brain-derived neurotrophic factor(BDNF) is a member of NGF family of neurotrophic factors. it is known that BDNF is involved in neurogenesis and synaptogenesis. Therefore BDNF is expected as biomarker for diseases of nervous system.In addition, it has been reported to be related to heart disease, diabetes, gout, periodontal disease, stress, exercise, etc., and it is the target of research in a wide range of fields.BDNF has precursor termed proBDNF, which is converted to mature BDNF(mBDNF) through the proteolytic removal of the N-terminal fragment by specific protease. It is reported that there are different functions between mBDNF and proBDNF. [Standard curve] 4.1-1,000pg/mL [Sample] human plasma and serum [Sample volume] 10uL [Assay time] 4 hour [Detection method] Colorimetric method |
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Manufacturer Information
Alias
- mBDNF ELISA Kit
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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