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Mature BDNF ELISA Kit Wako

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

SDS

Basic Information

Comparison
Product Number
Package Size
Price
Inventory
Distributor
296-83201
Barcode No
4548995079978
96Tests
List Price
759.00 USD

In stock

Document

SDS
Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Kit component

96 tests

Antibody-coated Plate 1 Plate
Mature BDNF Standard 100 μL
Buffer 60 mL
Biotin-conjugated Antibody Solution 100 μL
Peroxidase-conjugated Streptavidin Solution 100 μL
TMB Solution 6 mL
Stop Solution 6 mL
Wash Solution (10×) 100 mL
Plate Seal 4 Sheets

Features

  • High sensitivity
  • Detects mBDNF specifically
  • Small sample volume

Performance

03959167_img01.png
Standard curve range 4.1 - 1,000 pg/mL
Specificity mBDNF*
Sample Human serum and plasma**
Sample volume Human serum:10 μL Human plasma: 5 μL
Assay time 4 hours
Detection method Colorimetric method

* Approximately 10% reaction to proBDNF
** This antibody can also detect mouse and rat BDNF.

Assay principal

This kit is a sandwich ELISA using mBDNF monoclonal antibody and mBDNF polyclonal antibody. The kit has low cross-reactivity to proBDNF(approx. 10%) because the monoclonal antibody reacts with N-terminal cutting surface of mBDNF.

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Fujifilm Wako Company A Company B Company C
Cross-reactivity to proBDNF Approx. 10% Approx. 10% Approx. 15% Approx. 50%
Minimum concentration of
assay range
4.1 pg/mL 62.5 pg/mL 15.6 pg/mL 15.0 pg/mL

Data

Spiked recovery test

  • Sample Spike mBDNF (pg/mL) Assay value (pg/mL) Recovery volume (pg/mL) Recovery (%)
    Human serum
    0 330 - -
    100 423 93.0 93.0
    250 581 251 100
    500 817 487 97.4
    Human serum
    0 56.6 - -
    50 106 49.4 98.8
    100 154 97.4 97.4
    200 257 200 100
    300 363 306 102
  • Sample Spike mBDNF (pg/mL) Assay value (pg/mL) Recovery volume (pg/mL) Recovery (%)
    Human plasma
    0 156 - -
    100 252 96.0 96.0
    250 407 251 100
    500 661 505 101
    Human plasma
    0 66.0 - -
    50 115 49.0 98.0
    100 164 98.0 98.0
    200 276 210 105
    300 369 303 101

Dilution linearity

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Example of measurement -clinical sample -

Their serum mBDNF levels were measured by this kit. The samples were from healthy controls and patients with major depression, bipolar disorder, or schizophrenia.

  • Healthy control (n=6)
    No. Sex Age Assay value(ng/mL)
    1 F 48 14.5
    2 F 50 22.8
    3 F 54 20
    4 F 47 18.5
    5 F 42 13.8
    6 F 60 19.1
  • Major depression (n=6)
    No. Sex Age Assay value (ng/mL)
    1 F 66 15.7
    2 F 38 18.1
    3 F 43 10.0
    4 F 52 16.6
    5 F 41 22.3
    6 F 58 15.1
  • Bipolar disorder (n=4)
    No. Sex Age Assay value (ng/mL)
    1 F 46 16.5
    2 F 52 20.9
    3 F 52 11.5
    4 F 52 11.5
  • Schizophrenia (n=4)
    No. Sex Age Assay value(ng/mL)
    1 F 36 17.9
    2 F 53 23
    3 F 55 14.6
    4 F 51 10.8

Healthy person Major depression Bipolar disorder Schizophrenia
18.1 ± 3.41 16.3 ± 4.02 15.1 ± 4.53 16.6 ± 5.17

                                       (ng/mL)

Overview / Applications

Outline This item is designed for the quantitative detection and measurement of mature BDNF in biological fluids such as serum, and plasma. Brain-derived neurotrophic factor(BDNF) is a member of NGF family of neurotrophic factors. it is known that BDNF is involved in neurogenesis and synaptogenesis. Therefore BDNF is expected as biomarker for diseases of nervous system.In addition, it has been reported to be related to heart disease, diabetes, gout, periodontal disease, stress, exercise, etc., and it is the target of research in a wide range of fields.BDNF has precursor termed proBDNF, which is converted to mature BDNF(mBDNF) through the proteolytic removal of the N-terminal fragment by specific protease. It is reported that there are different functions between mBDNF and proBDNF.

[Standard curve] 4.1-1,000pg/mL
[Sample] human plasma and serum
[Sample volume] 10uL
[Assay time] 4 hour
[Detection method] Colorimetric method

Property

Manufacturer Information

Alias

  • mBDNF ELISA Kit

Product content may differ from the actual image due to minor specification changes etc.

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