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DNA ExtractorR SP kit

for Genetic Research
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
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SDS
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Distributor
296-60501
Barcode No
4987481439870
50Tests
List Price
275.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Application

Overview / Applications

Outline This product is for research use only. Do not administer it to human.DNA extraction from serum and plasma

DNA Extractor SP Kit efficiently extracts DNA fragments contained in serum and plasma. Based on the Sodium Iodide (NaI) method1, this kit enables the whole procedures done in a single micro-centrifuge tube without using hazardous phenol/chloroform.Recently, tumor-specific genes have been amplified and detected in the serum and plasma of patients with various diseases such as lung, breast and colon cancer. Many of these reference articles are currently being published2,3,4.The kit is a powerful pretreatment reagent for the detection and analysis of target DNA because of its high quality and yield.

Features

  1. High DNA yield (about 100%) from small amount of serum and plasma
  2. The DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA.
  3. Safe operation No phenol or chloroform required.
  4. Minimum contamination: The entire extraction of DNA can be done in a single centrifuge tube
  5. Complete removal of lipid derived from blood
  6. Less variability in extraction from sample to sample
Kit Contents (50 reactions)
  1. Enzyme reaction solution 1 x 10 mL
  2. Protein digestion solution 1 x 250 uL (uL: micro-L)
  3. Sodium iodide solution 1 x 15 mL
  4. Alcohol solution 1 x 30 mL
  5. Washing Solution (A) 1 x 50 mL
  6. Washing Solution (B) 1 x 50 mL
References
  1. Ishizawa, M., Kobayashi, Y., Miyamura, T. and Matsuura, S.: Nucleic Acids Res., 22, 1774 (1994).
  2. Sozzi, G., Musso, K., Ratcliffe, C., Goldstraw, P., Pierotti, M. A. and Pastorino, U.: Clin. Cancer Res., 5, 2689 (1999)
  3. Silva, J. M., Dominguez, G., Garcia, J. M., Gonzalez, R., Villanueva M. J., Navarro, F., Provencio, M., San, Martin, S., Espana, P. and Bonilla, F.: Cancer Res., 59, 3251 (1999)
  4. Shao, Z. M., Wu, J., Shen Z. Z. and Nguyen, M.: Clin. Cancer Res., 7, 2222 (2001)
Features 1.High DNA yield from small amount of serum and plasmaThe DNA extraction procedure is based on a simple centrifugal separation using Sodium Iodide and alcohol, that results in less loss of DNA than in solid-phase2. Safety of operation - Hazardous phenol and chloroform are not employed as reagents3. Minimizing the cross and extraneous contaminationThe entire extraction of DNA is done in a single centrifuge tube.4. Complete removal of lipids derived from blood using a specialized alcohol solution.5. Extraction of high quality DNA without PCR inhibition
Instructions [100 uL (200 uL)*1) of serum or plasma*2)] + 200 uL (300 uL) of Enzyme Reaction Solution and mix briefly + 5 uL (8-10 uL)*3) of Protein Digestion Solution and vortex Incubate at 56 degrees for 10 min. (at 56 degrees for 30 min.) + 300 uL (400 uL) of Sodium Iodide Solution and mix briefly + 600 uL (900 uL) of Alcohol Solution and vortex Incubate at R.T. for 10 min. Centrifuge at 12~20K x g at R.T. for 10 min.[Pellet]*4) + 1 mL (1.5 mL)*5) of Washing Solution (A) to the pellet and vortex*6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]*4) + 1 mL (1.5 mL) of Washing Solution (B) to the pellet and vortex*6) Centrifuge at 12~20 K x g at R.T. for 5 min.[Pellet]*4) Dry the pellet well for about 5 min. at 65 degrees C + Adequate volume (10~20 uL) of TE (pH 8.0) or D.W. (10~20 uL) and vortex*6) Dissolve the pellet completely with vortex mixer and incubate at 65 degrees C for 3~5 min.*7)[DNA Solution]: Store at 20 degrees C.

Note:uL: micro litter*1) Grouping symbol means the protocol from 200 uL of sample.*2) Samples of serum and plasma should be placed on ice for the avoidance of activation of the endogenous DNase. We recommend Enzyme Reaction Solution to be added as soon as possible.*3) Protein Digestion Solution should be placed on ice, during operation.*4) Decant and remove the supernatant as much as possible from the tube that is inverted and placed on paper towel.*5) It is possible to store the solution at - 20 degrees for long term.*6) Mix vigorously until the pellet is removed from wall of tube.*7) Dissolve the pellet completely with vortex mixer and incubation.

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For research use or further manufacturing use only. Not for use in diagnostic procedures.

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