DNA ExtractorR WB Kit (Sodium Iodide method)
- for Whole Blood DNA Extraction
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at 2-10 degrees C.
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50Tests
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In stock in Japan |
※Check availability in the US with the distributor.
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| Outline | This product is for research use only. Do not administer it to human. The DNA Extractor WB Kit extracts genomic DNA from whole blood, cell culture, and tissue. It employs a new extraction procedure for DNA purification after the lysis of cells. This procedure, using Sodium Iodide (NaI) as a chaotropic agent, realizes intact DNA isolation of both high purity and high yield. Furthermore, extraction is done through several brief microfuge centrifugations from a single microfuge tube. DNA extracted with this kit is suitable for several applications, including restriction enzyme digestion, Southern blot analysis, and PCR. This kit is applicable to 100-1000uL of whole blood by proportionally varying the volume of solutions used in the standard method described below (1 mL of whole blood can be applied by using a 2 mL microcentrifuge tube and doubling the amount of each reagent). Whole blood stored refrigerated for 3 weeks did not show a significant decrease in the recovery of DNA with this kit. Ref.: Wang, L., Hirayasu, K., Ishizawa, M., and Y. Kobayashi, ''Purification of genomic DNA from human whole blood by isopropanol-fractionation with concentrated NaI and SDS'', Nuclei Acids Research, 22(9), 1174-75 (1994). |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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