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Quick-CBB PLUS

for Electrophoresis
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
178-00551
Barcode No
4987481436350
1L
List Price
127.00 USD

In stock in Japan

Distributor
174-00553
Barcode No
4987481436435
250mL
List Price
48.00 USD

In stock in Japan

Document

SDS
Product Specification Sheet
Spectral Data
Certificate of Analysis
Calibration Certificate

Application

Overview / Applications

Outline Quick-CBB Plus is astain solution supplied in a bottle, and can quickly and easily stain a protein band of polyacrylamide gel. Unlike conventional Quick-CBB, fixation is not needed, and organic solvents such as methanol and acetate are not used.Since the solution is supplied in a bottle, mixing operation is unnecessary. The background is set lower for better use.Like conventional Quick-CBB, decoloring operation is unnecessary.
Protein Research / Simple SDS-PAGE Gel StainingQuick-CBB PLUS is a bottled protein staining kit, which allows simple and quick staining of proteins bands in polyacrylamide slab gels. In comparison with conventional Quick-CBB, fixing procedure is not required and organic solvents such as methanol and acetic acid are not used. The mixing procedure is now also unnecessary as all the required solutions for staining are contained in a single bottle. There are also other improvements such as coloration not occurring on the background. As in conventional Quick-CBB, destaining procedure is not required.

[Features]

  • No fixing procedure and no organic solvents are necessary.
  • Simple and quick staining without the coloration on background
Quick-CBB PLUS Staining Protocol
  1. WashingWash gel 2 times with deionized water <5 minutes x 2 times>
  2. StainingStain gel by adding Quick-CBB PLUS
  3. WashingWash gel for 1 hour with deionized water

[FAQ]

  1. Q: When protein bands are detected a few minutes after the start of staining, can the staining procedure be terminated by washing the gel?A: Quick-CBB PLUS usually stains protein bands in 10-20 minutes. The staining can be terminated at that time, but 30-60 minutes is recommended for proper staining.
  2. Q: When protein bands can be clearly detected and background staining does not occur without washing after staining, is the washing procedure still required?A: The washing procedure is not imperative, but a clearer staining can be obtained by washing the gel. When protein bands are light after 60-minute of washing, a clearer staining can be obtained after overnight washing.
  3. Q: About disposalA: Quick-CBB PLUS does not contain any harmful substances, but is a very dark blue color. Therefore it should be collected in a container prepared for disposal.
  4. Q: As for the sensitivity of the stainingA: Some protein bands visible to approx. 10 ng

This product is for research use only. Do not administer it to human.

Property

Appearance Blue liquid

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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