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Microglia Remover

for Cellbiology
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
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Certificate of Analysis
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Distributor
134-19501
Barcode No
4548995100023
1mL
List Price
385.00 USD

In stock in Japan

Document

Product Specification Sheet
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Product Overview

Microglia Remover is anionic liposomes suspension containing clodronic acid. When this product is phagocytosed by microglia as foreign substances, clodronic acid is released within the cells, causing cell death. By encapsulating clodronic acid in liposomes, it is taken up into microglia more efficiently than clodronic acid alone. Microglia remover (for Control) (sold separately, Product Number 131-19511) is also available as a control for experiments.

Features

  • Microglia can be removed in approximately 1-3 days after administration
  • Less expensive than commercial microglia removal feeds

Product Information

Lipid composition Dipalmitoylphosphatidylcholine, Dipalmitoylphosphatidylserine and Cholesterol
Average particle size 100 nm
Formulation Phosphate Buffered Saline sterilized with 0.22 μm filter

Protocol

Intracerebral infusion

  1. 1-2 μL Microglia Remover or Microglia Remover (for Control) are infused into the cerebral parenchyma.
  2. Assess the depletion of microglia in 1-3 days post-infusion.

Data

Application Data

Microglia removal in mouse cerebral cortex

After inserting a 33 G needle into the mouse cerebral cortex, 1 µL of Microglia Remover (for Control) or Microglia Remover (this product) was injected, respectively, and sampled after 3 days.

Control
Microglia Remover(for Control)sample image
Microglia Remover (This product)
Microglia Remover sample image
Animal
Mouse (C57BL6/J)
Site
Cerebral cortex
Sample
Frozen section
Antibody Concentration
Primary Antibody
Anti Iba1, Rabbit (for immunocytochemistry) (Product Number 019-19741) 1:500
Secondary Antibody
Alexa Fluor® 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (Jackson ImmunoResearch Product Number 111-545-144) 1:500
Data by courtesy of
Dr. Miyata, Department of Applied Biology, Kyoto Institute of Technology
[Result]
In the control group, microglia (or macrophages) accumulated in large numbers at the site of injury.
On the other hand, the “Microglia Remover” group showed inhibition of the accumulation of microglia in the injured area and observed a decrease or regression in the number of microglia in the surrounding area.

FAQ

About usage

Are there any precautions when using the product?
Clodronate liposomes may precipitate after long-term storage. slowly invert the vial several times until the suspension appears homogeneous by visual inspection.

Are there any precautions for storage?
Avoid freezing and close the cap of the tube to prevent an influence on liposomes caused by oxidation.

About application

Is it possible to remove macrophages?
It is known that clodronate liposomes can also remove macrophages, which may be possible in principle, but we have not conducted any experiments.

Overview / Applications

Outline Microglia Remover is anionic liposomes suspension containing clodronic acid. When this product is phagocytosed by microglia as foreign substances, clodronic acid is released within the cells, causing cell death. By encapsulating clodronic acid in liposomes, it is taken up into microglia more efficiently than clodronic acid alone.


Properties :Liquid
Average particle size : 100 nm
Buffer :PBS
Application
1. 1~2 uL Microglia Remover or Microglia Remover (for Control) are infused into the cerebral parenchyma.
2. Sampling is performed 1~3 days after injection and evaluated by immunohistochemistry or other methods.
Precautions for Use 1. Avoid freezing.
2. If it is not used for a long period of time, slowly invert the vial several times until the suspension appears homogeneous by visual inspection.
3. When using Microglia Remover, take a sample from this product with sterilized instrument.
4. Close the cap of the tube to prevent an influence on liposomes caused by oxidation.

Property

Appearance Liquid

Manufacturer Information

Alias

  • Microglia Removal Reagent

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.