BES-Thio

Biochemistry
Highly Selective Fluorescent Probes
Thiol / Serenol selective fluorescent probe
Several chemiluminescent and fluorescent reagents are known as a thiol group detection reagent, and used for detection of thiol groups or measurement of cholinesterase activity.
Most of these reagents have a low hydrophilicity, so separate reaction steps, enzyme and detection reactions, are required.BES-Thio has a high hydrophilicity and can be used in aqueous solution. This feature makes it easy to measure the enzyme activity such as cholinesterase using acetylthiocholine or butyrylthiocholine as a substrate. Furthermore, by pH adjustment, BES-Thio can also detect serenol groups, which sulfur (S) in thiol group is substituted by selenium (Se), and can be used for selenoprotein detection reagent.
[Features]

1. High hydrophilicity


2. Respond to thiol at pH 7.4


3. Respond to serenol at pH 5.8

1. Prepare 5 mM ethanol solution for the Stock Solution.


2. Probe Solution is prepared by the Stock Solution diluted with appropriate buffer be diluted by 200-fold.


3. The probe solution can be used by mixing with Enzyme-Substrate Reaction solution, etc.

e.g.)
[Cholinesterase activity determination using 96-well microplate]
Mix 10 uL of Enzyme-Substrate Reaction solution and 170 uL of Probe Solution and incubate the solution for a certain time.
Then measure generated fluorescence.
(uL: microliters)

Detection Wavelength
Excitation wavelength: 495 nm
Emission wavelength: 535 nm

1. Maeda, H., Matsuno, H., Ushida, M., Katayama, K., Saeki, K. and Itoh, N.: Angew. Chem. Int. Ed., 44, 2922 (2005).


2. Maeda, H., Katayama, K., Matsuno, H. and Uno, T.: Angew . Chem. Int. Ed., 45, 1810 (2006).


3. Maeda, H: Wako Jun-yaku Jiho, 73 (3), 2 (2005) (written inJapanese).

• BES-Thio (for Thiol or Serenol ) [Fluorescence]excitation wavelength: 495 nm; emission wavelength: 535 nm (Wako Cat. #025-15481 (1 mg))
• BES-So (for Superoxide) [Fluorescence]excitation wavelength: 505 nm; emission wavelength: 544 nm (Wako Cat. #021-15601 (1 mg))
• BES-H₂O₂ (for Hydrogen Peroxide) [Fluorescence] excitation wavelength: 485 nm; emission wavelength: 515 nm (Wako Cat. #029-15381 (1 mg))

The product information is shown on the page #4 of Wako Product Update No.17.