Anti Claudin-5, Rat Monoclonal Antibody(R9)
- for Immunochemistry
- Manufacturer :
- FUJIFILM Wako Pure Chemical Corporation
- Storage Condition :
- Keep at -80 degrees C.
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500uL
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In stock in Japan |
※Check availability in the US with the distributor.
Document
Antibody information
| Anti Claudin-5, Monoclonal Antibody (R9) | |
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| Code No. | 014-28101 |
| Clone No. | R9 |
| Antigen | Vector expressing human claudin-5 full length accession#AAH19290 |
| Buffer | PBS |
| Subclass | Rat IgG2b・κ |
| Cross-reactivity | Human, monkey |
| Specificity | Claudin-5 No cross-reaction to Claudin-1-4,6,71) |
| Concentration | Indicated to the label First lot：1.1mg/mL |
| Application | Flow cytometry 0.5-5μg/mL Neutralizing assay(in vitro) 90-150μg/mL |
Application Data
Neutralizing assay in vitro BBB cell model
The barrier activity was evaluated by adding these antibodies (R9 and M48) were added to the blood-brain barrier cynomolgus cell model (BBB kit, PharmaCo-cell).
| (1)Trans-epithelial/endothelial electrical resistance (TEER) | (2) Substance permanent |
| These antibodies(R9 and M48) reduced TEER. The effects were higher than C-CPEmt. |
These antibodies(R9 and M48) increased permanent of fluorescein isothiocyanate–labeled dextran(4kDa).The effects were higher than C-CPEmt. |
Overview / Applications
| Outline | Claudin-5 is a tetra-transmembrane protein with approximately 23kDa, belongs to a member of Claudin family. It functions as a key component in blood-brain barrier. This product is a rat monoclonal antibody which specifically recognizes Claudin-5, and has neutralizing activity have neutralizing activity which reduces barrier function in blood-brain barrier model. This product is sold under thelicense of Osaka University-Ehime University-FUJIFILM Wako Pure Chemical Corporation joint application patent. PCT/JP2018/016975 [Anti-cldn-5 antibody, and drug containg said antibody] [Antibody subclass] rat IgG2b [Cross-reactivity] human, monkey [Applicatin] neutralizing assay(in vitro), flow cytometry [Reference] 1) Hashimoto, Y., et al.: J. Pharmacol. Exp. Ther., 363(2), 275(2017). |
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Manufacturer Information
Alias
- Anti CLDN5, Rat Monoclonal Antibody(R9)
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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