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Anti Mouse Nestin, Rat Monoclonal Antibody(7A3)

for Immunochemistry
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at -20 degrees C.
Source :
Mouse
Host :
Rat
Clone No. :
7A3
Classification :
Monoclonal
GHS :
  • Structural Formula
  • Label
  • Packing
SDS
Comparison
Product Number
Package Size
Price
Inventory
Distributor
012-26843
Barcode No
4548995058553
50uL
List Price
330.00 USD

In stock in Japan

Document

SDS
Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate

Application data

Immunocytochemistry

03316943_img01.png
Left: Nestin, Middle: Hoechst 33342 (nucleus), Right: Phase contrast image
Experimental conditions
Sample Mouse neural precursor cells
Antibody concentration 10 µg/mL
Secondary antibody Anti-rat IgG-Alexa Fluor 488

Cortex-derived neural stem cells were stained positive.

immunohistochemistry

03316943_img02.png
Left/ green: Nestin, Middle/ blue: Hoechst 33342 (Nucleus)
Experimental conditions
Sample Cerebral cortex from mouse on embryonic day 12.5
Antibody concentration 10 µg/mL
Secondary antibody Anti-rat IgG-Alexa Fluor 488

Neural stem cells expressing Nestin were identified in the cerebral cortex from mouse on embryonic day 12.5.

Overview / Applications

Outline This product is for research use only. Do not administer it to human.
Nestin is intermediate dilament protein and belongs to a class VI intermediate filament. The protein is commonly known neural stem cells and neural progenitor cells marker in developmen of the central nervous system. This product is a rat moniclonal antibody that reacts with Nestin.
[Reactivity] Reacts with Nestin
[Antigen] mouse E16 embryonic cerebral cortex extracts
[Species cross reactivity] mouse
[Clone No.] 7A3
[Host] Rat
[Isotype] IgG2b kappa
[Concentration] Indicated on the label.
[Formulation] PBS with 50% Glycerol and 0.05% ProClin300
[Applications]
ICC 1:100-500
IHC 1:100-500

Property

Appearance Liquid

Antibody Information

Antigen Nestin
Antigen Alias Nestin
NES
FLJ21841
NBLA00170
Subclass IgG2b,k
Labeling Unlabeled

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

Product content may differ from the actual image due to minor specification changes etc.

If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.

877-714-1920

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