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Detail Information
  1. Top
  2. Life Science
  3. Protein Research
  4. Protein Phosphorylation Analysis
  5. Phos-tag™ Agarose
  6. Phos-tag™ Agarose

Phos-tag™ Agarose

Manufacturer :
NARD INSTITUTE, LTD.
Storage Condition :
Keep at 2-10 degrees C.
GHS :
  • Structural Formula
  • Label
  • Packing
Comparison
Product Number
Package Size
Price
Availability
Certificate of Analysis
Purchase
Distributor
389-21201
Manufacturer
AG2-501
0.5mL
Inquire

In stock in Japan
Distributor
Distributor
385-21203
Manufacturer
AG2-503
3mL
Inquire

In stock in Japan
Distributor

※Check  availability  in the US with the distributor.

Document

Package Insert
Spectral Data
Certificate of Analysis
Calibration Certificate
Analytical Charts

Overview

04545184_img01.png

This product is agarose bound to Phos-tagTM, a functional molecule that traps phosphate by having two divalent metal ions.
Phosphoproteins can be separated, purified, and concentrated from the mixture by filling the column. Since surfactants and reducing agents are not used, phosphoproteins in a state close to that in vivo can be obtained.
This product is a renewed product with less nonspecific binding than the previous products (302-93561, 308-93563) by adjusting the binding amount of Phos-tagTM functional group.

04545184_img02R.png

Features

  • Phosphoproteins can be purified in less than 1 hour.
  • The proteins can be trapped in physiological condition (pH 7.5).
  • Purified without reducing agent and surfactant.

Application ~Purification of phosphorylated proteins in A431 lysate ~

  1. Phos-tagTM Agarose was filled into the column and A431 cell lysate was appliqued onto it.
  2. Elution fractions obtained after adsorption, washing, and elution operations were separated by SDS-PAGE along with cell lysate and flow-through/wash fractions.
  3. Fluorescent staining (detecting all proteins) and Phos-tagTM biotin confirmed that phosphoproteins were enriched in the eluted fraction.
04545184_img03.png

Manufacturer Information

Alias

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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