Oligonucleotide Synthesis

Oligonucleotides are generally synthesized by using a solid-phase synthesis called "phosphoramidite method", in which amidites are used. The following cycle is repeated until the desired chain length is reached: nucleosides fixed on a support >> detritylation >> coupling reaction of amidites >> cap reaction >> oxidation (or sulfidization) reaction. This reaction is performed using the automatic synthesis device. Furthermore, FUJIFILM Wako deal with all reaction supporting reagents that are related to the above-mentioned synthesis of oligonucleotides.

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