Capping
In the process for phosphoramidite method, capping reaction after coupling reaction is important in order to avoid generating by-products. Before next step (oxidation) is carried out, unreacted nucleotide/oligonucleotide that is starting material at coupling reaction should be removed by the reaction. Because, by-products after oxidation step make a complicated for purification step when unreacted material remained.
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Acetonitrile solution
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Activators, Solution
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Deblocking
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