TNF-α ELISA Kits

TNF-α (Tumor necrosis factor-α) is an inflammatory cytokine, originally identified as a factor that induces hemorrhagic necrosis in tumors transplanted into mice. Studies have shown that TNF-α activates various immune functions and exerts antimicrobial and antitumor effects through the induction of apoptosis¹⁾.
TNF-α is produced by a variety of cells, including activated macrophages, B cells, T cells, and fibroblasts. Membrane-bound TNF-α, a type II transmembrane cell surface polypeptide, serves as the precursor, and soluble TNF-α is generated through cleavage by enzymes such as TNF-α converting enzyme (TACE). Soluble TNF-α binds to two receptors (TNFR1 and TNFR2) and exerts biological activities such as induction of apoptosis and enhancement of antibody production²⁾. TNFR1 is ubiquitously expressed on virtually all cell types, while TNFR2 is expressed primarily on immune cells. TNF-α is also known to induce activation of the transcription factor NF-κB, a transcription factor that translocates to the nucleus and regulates gene transcription³⁾.
TNF-α secretion is upregulated in enlarged adipocytes, where it inhibits insulin action and contributes to insulin resistance, making it a focus of research in glucose metabolism. It has also been implicated in numerous diseases, including rheumatoid arthritis, inflammation, diabetes, hyperlipidemia, nephropathy, sepsis, osteoporosis, Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis (ALS).

LBIS™ Human TNF-α ELISA Kit is a sandwich ELISA kit for the quantitative measurement of human TNF-α. This kit enables highly sensitive quantification of TNF-α in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Measurement of TNF-α in Human Serum and Plasma

N.D.: Below the lower limit of quantification

[Result]
This product was able to detect TNF-α even in samples that were below the lower limit of quantification of another manufacturer's kit.

LBIS™ Mouse TNF-α ELISA Kit is a sandwich ELISA kit for the quantitative measurement of mouse TNF-α. This kit enables highly sensitive quantification of TNF-α in biological samples. The standard included in the kit is a recombinant protein produced in Escherichia coli.

Measurement of TNF-α in Mouse Serum and Plasma

N.D.: Below the lower limit of quantification

[Result]
This product was able to detect TNF-α even in samples that were below the lower limit of quantification of another manufacturer's kit.

1. Okada, M.: Journal of JCS Cardiologist, 7(2), 259(1999). (Japanese)
   tumor necrosis factor-α : TNF-α
2. Horiuchi, T. et al.: Rheumatology., 49(7), 1215(2010).
   Transmembrane TNF-α : structure, function and interaction with anti-TNF agents
3. Horiguchi, H. et al.: The Journal of Showa University Dental Society, 26(2), 125(2006). (Japanese)
   The Role of NF-κB in TNF-α-Induced Apoptosis in Human Chondrocyte-Like Cells (USAC)