Synthetic Sweeteners

Sweeteners, include natural sweeteners, prepared/concentrated by extracting sweet components found in natural plants/foods, and synthetic sweeteners (artificial sweeteners, sugar alcohol), artificially synthesized. Synthetic sweeteners, such as dulcin and sodium cyclamate (called Chikuro), were widely used in Japan. These are now prohibited due to their carcinogenicity but are sometimes detected in imported foods.
FUJIFILM Wako provide a variety of reagents for analyzing synthetic sweeteners in food.

Analysis Examples (HPLC)

Acesulfame K

It is known as acesulfame potassium, and has 200-250 times the sweetness of sugar. As a high-sweetness sweetener, it is added to sugar substitute sweeteners in combination with xylitol, soft drinks, confectionery, and pickles.

Aspartame

It is about 200 times as sweet as sugar and is used in foods such as diet sweeteners, soda, yogurt, and ice candies.

Advantame

It is a dipeptide methyl-ester derivative synthesized by reducing the alkylation of aspartame, a sweetener, with 3-hydroxy-4 methoxy-phenylpropionaldehyde. Although sweetness depends on the type of food in which it is used and blending composition, its sweetness is approximately 14000-48000 times that of sugar and 90-120 times that of aspartame.


  • 01816_img08.png
  • HPLC Conditions
    Sample Advantame Standard
    Column Wakosil-Ⅱ 5C18HG, 4.6 mmφ × 250 mm
    Eluent A:phosphate Buffer(pH 2.8) 900 mL+CH3CN 100 mL
    B:phosphate Buffer(pH 2.8) 400 mL+CH3CN 600 mL
    0-30 min. B:15%, 30-55 min. B:15-25%
    55-75 min. B:25-100%, 75-90 min. B:100%
    Flow rate 1.0 mL/min. at 50℃
    Detection UV 210 nm
    Injection vol. 1 mg/mL soln. 20 μL

Advantamame Acid

It is a hydrolysate of advantame and is considered a major metabolite.


  • 01816_img09.png
  • HPLC Conditions
    Sample Advantame Acid Standard
    Column Wakosil-Ⅱ 5C18HG, 4.6 mmφ × 250 mm
    Eluent A:phosphate Buffer(pH 2.8) 900 mL+CH3CN 100 mL
    B:phosphate Buffer(pH 2.8) 400 mL+CH3CN 600 mL
    0-30 min. B:15%, 30-55 min. B:15-25%
    55-75 min. B:25-100%, 75-90 min. B:100%
    Flow rate 1.0 mL/min. at 40℃
    Detection UV 280 nm
    Injection vol. 0.2 mg/mL soln. 10 μL

Dulcin

This artificial sweetener was developed in Germany in 1883. With a sweetness of about 200 - 400 times that of sucrose, it was widely distributed in Japan after the war. However, addition of the sweetener to foods was completely prohibited in January 1969 in Japan, following intoxication accidents and detection of toxicity causing hepatic dysfunction and carcinogenicity.
Currently, dulcin is not used globally. However, dulcin was detected from foods imported; therefore, inspections are performed at quarantine stations.

Saccharin Sodium Dihydrate

This artificial sweetener is about 500 times as sweet as sugar in aqueous solution. Sweeteners that are currently allowed in drinks and foods include saccharin sodium dihydrate and aspartame, which was approved in August 1983 in Japan. Saccharin sodium dihydrate can also inhibit cholinesterase activity.

Sucralose

Sucralose, developed in 1976, is approximately 600 times sweeter than sugar and is used as a raw material in foods and beverages.

Cyclamic Acid Sodium Salt

This synthetic sweetener was permitted in the past, but in November 1969, the use of this synthetic sweetener was prohibited in Japan. However, the use of sodium cyclamate is approved in the EU, China, Taiwan, etc. and it is frequently detected at quarantine stations.

Analysis Examples (TLC)

Analysis of artificial sweeteners using the Ninhydrin-ethanol TS Spray

Target compound
01816_img10.png 01816_img11.png 01816_img12.png
1. Glutamyl–valyl–glycine 2. Alitame 3. Advantame

  • 01816_img13.png
  • Analysis conditions
    Deployed solvent Chloroform/Methanol/Acetic acid/Water = 30/20/4/6
    Concentration of mixed solution 1. 10 μg/μL, 2. 2.5 μg/μL, 3. 2.5 μg/μL
    Development distance 7 cm (saturation development)
    Injection volume 4 μL
    TLC plate Silicagel 70 TLC Plate-Wako

Product List

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Standards

Acesulfame K Standard

for HPLC

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
019-19481
500mg
List Price
69.00 USD

In stock in Japan

Aspartame Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
017-22611
100mg
List Price
77.00 USD

In stock in Japan

Advantame Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
018-26801
100mg
List Price
131.00 USD

In stock in Japan

Advantame Acid Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
015-26811
100mg
List Price
124.00 USD

In stock in Japan

Dulcin Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
043-31021
100mg
List Price
124.00 USD

In stock in Japan

Saccharin Sodium Dihydrate Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
195-15551
100mg
List Price
65.00 USD

In stock in Japan

Sucralose Standard

for HPLC

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
198-12241
1G
List Price
88.00 USD

In stock in Japan

Cyclamic Acid Sodium Salt Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.
GHS :

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
035-21031
100mg
List Price
101.00 USD

In stock in Japan

Analytical column

Wakopak Wakosil-II5C18HG 4.6*150mm
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
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Package Size
Price
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Purchase
Distributor
238-51111
1Column(D)
List Price
556.00 USD

Out of Stock

Distributor
234-51113
1Column(W)
List Price
556.00 USD

In stock in Japan

Wakopak Wakosil-II5C18HG 4.6*250mm
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
232-51131
1Column(D)
List Price
592.00 USD

In stock in Japan

Distributor
238-51133
1Column(W)
List Price
592.00 USD

In stock in Japan

Wakopak Navi C18-5 4.6*250mm
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
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Package Size
Price
Availability
Purchase
Distributor
235-60531
1Column(D)
List Price
592.00 USD

Out of Stock

Distributor
231-60533
1Column(W)
List Price
592.00 USD

Out of Stock

WakopakR Ultra C18-5 Φ4.6mm × 250mm (W)
Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
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Price
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Purchase
Distributor
232-02661
1EA(W)
List Price
726.00 USD

In stock in Japan

Related Product List

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Acesulfame K

for Biochemistry

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
013-14102
25G
List Price
48.00 USD

Out of Stock

Distributor
015-14101
100G
List Price
146.00 USD

Out of Stock

Aspartame

for Food Additives Test

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep below 25 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
016-11331
10G
List Price
54.00 USD

In stock in Japan

Glutamyl-Valyl-Glycine Standard

for Food Analysis

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at 2-10 degrees C.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
077-06391
100mg
List Price
121.00 USD

In stock in Japan

Saccharin Sodium Dihydrate

Wako Special Grade

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
193-08602
25G
List Price
22.00 USD

In stock in Japan

Distributor
197-08605
500G
List Price
85.00 USD

In stock in Japan

Silicagel 70 TLC Plate-Wako

for TLC

Manufacturer :
FUJIFILM Wako Pure Chemical Corporation
Storage Condition :
Keep at RT.

Comparison
Product Number
Package Size
Price
Availability
Purchase
Distributor
199-17874
10plates(Layer Thickness: 0.25mm, Size: 5cmx10cm)
List Price
42.00 USD

In stock in Japan

Distributor
195-17871
25plates(Layer Thickness: 0.25mm, Size: 20cmx20cm)
List Price
252.00 USD

In stock in Japan

Distributor
191-17873
100plates(Layer Thickness: 0.25mm, Size: 5cmx20cm)
List Price
365.00 USD

In stock in Japan

For research use or further manufacturing use only. Not for use in diagnostic procedures.

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