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Amino Acids for SILAC

Stable isotope labeling using amino acids in cell culture (SILAC) can be used to identify and quantify proteins in complex protein samples for comparison of expression levels. Stable isotope-labeled amino acids are metabolically introduced into proteins during cell culture to prepare samples for protein identification and quantification by mass spectrometry. FUJIFILM Wako provides four 13C- or 15N-labeled basic amino acids.

Examples of specifications

Products Amino Acids L-Arginine Hydrochloride L-Lysine Monohydrochloride
Labeling 13C6, 15N4 13C6 13C6, 15N2 13C6
Requirements Specifications
Appearance White to light yellow crystalline powder to powder White to slightly light yellow crystals to powder or aggregate
13C conversion rate ≥99 atom% ≥98 atom%
15N conversion rate ≥99 atom% ≥99 atom%
Thin-layer chromatography test Meets the test requirements (purity ≥99%)

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Staining Reagents for MS

In-gel Digestive Enzyme

High Purity Matrix

Calibrants for MALDI-MS

Product content may differ from the actual image due to minor specification changes etc.

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