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Protein Assay Bradford Reagent

This product is a solution containing Coomassie Brilliant Blue (CBB) G-250, which is used to measure protein concentrations in solution using the Bradford method. When CBB binds to a protein under acidic conditions, the maximum absorption wavelength shifts from 465 to 595 nm. This change in absorbance can be used to determine protein concentrations in solution. Surfactant in the measurement sample does not affect the measurement if the concentration is below a certain level (see the effects of coexisting substances, described below).

Examples of use according to the measurement range

Examples of measurement results (1) (2) (3)
Instruments used test tube test tube microplate
Measurement range 100 ~ 1,000 µg/mL 6.25 ~ 100 µg/mL 0.78 ~ 25 µg/mL
Volume of measurement sample 10 µL 20 µL 150 µL
This product 500 µL 380 µL 150 µL
Reaction time Stand at room temperature for 10 min
Measurement wavelength 595 nm
  • Please consider the mixing ratio of sample and this product according to the measurement range.

Examples of measurement results

Effects of coexisting substances

When measuring protein concentrations using this product, it has been demonstrated that none of the following substances in the measurement sample affect measurement results if the concentration is less than or equal to the indicated concentration:

Substance Concentration
CHAPS 5 %
Triton X-100 0.1 %
Tween 20 0.1 %
SDS 0.025 %
NP-40 0.1 %
Ethanol 10 %
Glycerol 20 %
Methanol 10 %

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Protein Determination Reagents

Protein Standard

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