Protein Assay Bradford Reagent
This product is a solution containing Coomassie Brilliant Blue (CBB) G-250, which is used to measure protein concentrations in solution using the Bradford method. When CBB binds to a protein under acidic conditions, the maximum absorption wavelength shifts from 465 to 595 nm. This change in absorbance can be used to determine protein concentrations in solution. Surfactant in the measurement sample does not affect the measurement if the concentration is below a certain level (see the effects of coexisting substances, described below).
Examples of use according to the measurement range
| Examples of measurement results | （１） | （２） | （３） |
|---|---|---|---|
| Instruments used | test tube | test tube | microplate |
| Measurement range | 100 ～ 1,000 µg/mL | 6.25 ～ 100 µg/mL | 0.78 ～ 25 µg/mL |
| Volume of measurement sample | 10 µL | 20 µL | 150 µL |
| This product | 500 µL | 380 µL | 150 µL |
| Reaction time | Stand at room temperature for 10 min | ||
| Measurement wavelength | 595 nm | ||
- Please consider the mixing ratio of sample and this product according to the measurement range.
Examples of measurement results
Effects of coexisting substances
When measuring protein concentrations using this product, it has been demonstrated that none of the following substances in the measurement sample affect measurement results if the concentration is less than or equal to the indicated concentration:
| Substance | Concentration |
|---|---|
| CHAPS | 5 % |
| Triton X-100 | 0.1 % |
| Tween 20 | 0.1 % |
| SDS | 0.025 % |
| NP-40 | 0.1 % |
| Ethanol | 10 % |
| Glycerol | 20 % |
| Methanol | 10 % |
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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