[NIPPON GENE] Agarose
NIPPON GENE provides agarose which is specially purified for the purpose of separation and recovery of DNA fragments. Sufficient separation and purity of recovered DNA are ensured by strict quality control.
Purpose and specification
| Product Name | S、S＜Tablet＞ | HS | L | H | XP | 21 | X |
|---|---|---|---|---|---|---|---|
| Feature/Application | Standard | Blotting, etc. | Recovering DNA | High gel strength | Recovering DNA, 3 % gel | 3% gel | 3% gel |
| Separation range (kbp) | 0.5 - 30 | 0.5 - 30 | 0.5 - 30 | 1 - 200 | 0.01 - 20 | 0.01 - 1.0 | 0.01 - 1.0 |
| Concentration range | 0.5 - 2% | 0.5 - 2% | 0.5 - 2% | 0.2 - 1% | 1 - 4% | 2 - 5% | 2 - 6% |
| No weighing required | 0.5 g/tablet*1 | 3g*2 | |||||
| Gel strength | ≥1,200g/cm2 (1.5%) |
≥1,600g/cm2 (1.5%) |
≥450g/cm2 (1.5%) |
≥2,600g/cm2 (1.5%) |
≥450g/cm2 (1.5%) |
≥800g/cm2 (3%) |
≥1,200g/cm2 (4%) |
| Melting point | ≤90°C (1.5%) |
≤93°C (1.5%) |
≤65°C (1.5%) |
boil (1.5%) |
≤65°C (1.5%) |
≤85°C (3%) |
≤92°C (4%) |
| Gelation temperature | 37～39°C (1.5%) |
37～39°C (1.5%) |
≤30°C (1.5%) |
37～39°C (1.5%) |
≤30°C (1.5%) |
34～38°C (3%) |
31～39°C (4%) |
*1 Agarose S
*2 Stick type (3 g) is also available for Agarose 21.
Quality control
- Gel strength
- Melting point
- Gelation temperature
- Sulfate content (SO4)
- Water content
- Electroendosmosis (-Mr)
- No detection of DNase and RNase
- Check for recovered DNA
- Restriction enzyme cleavage
- Ligation with T4 DNA ligase
- Transformation of E. coli with ligated DNA (e.g., pBR322)
Product List
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Agarose S
Agarose HS
Agarose L
Agarose H
Agarose XP
Agarose 21
Agarose X
For research use or further manufacturing use only. Not for use in diagnostic procedures.
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If the revision of product standards and packaging standards has been made, there is a case where the actual product specifications and images are different.