ELISA-Star™ Chemiluminescent Peroxidase Substrate
ELISA-Star™ Chemiluminescent Peroxidase Substrate is a highly sensitive chemiluminescent peroxidase reagent for ELISA. It has highly stable luminescence and excellent sensitivity and quantifiability in the low antigen concentration region.
Features
- Highly sensitive detection
- Prolonged luminescence
Kit composition
| Product Number | 299-78801 | 295-78803 | 293-78804 |
|---|---|---|---|
| Volume | For 20 mL | For 100 mL | For 250 mL |
| Luminescent solution A | 10 mL | 50 mL | 125 mL |
| Luminescent solution B | 10 mL | 50 mL | 125 mL |
Comparison of luminescence sensitivity
ELISA-Star™ has excellent quantifiability in the low concentration range.
Luminescence persistence
Luminescence decay rate
- ELISA-Star™ -22.0% after 10 min, -32.2% after 20 min (See below a figure)
- Competitor's product -34.0% after 10 min
Usage
- Prepare a well plate that has completed a peroxidase-labeled antibody reaction.
- Place and mix equal volumes of Luminescent solution A and Luminescent solution B in a tube.
- Allow the mixture to stand for approximately 15–30 minutes until it returns to room temperature.
- Add 100–150 µL of the mixture to each well of the plate.
- Shake the plate for 1 minute on a microplate shaker.
- Measure the luminescence using a luminometer.
- Measure the luminescence within 1–20 minutes after addition of the mixture to the well. As time passes, the intensity of the luminescence decreases.
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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