Achromopeptidase®
Achromopeptidase® is a Lysobacter enzymogenes-derived bacteriolytic enzyme. It has a broad bacteriolytic spectrum, is more bacteriolytic than egg-white lysozyme, and lyses a wide range of bacteria. It can also be used to prepare protoplasts such as actinomycetes, etc.
Product Information
Molecular weight: Approximately 20,000
pH stability: Most stable at pH 6.0 and almost stable at pH 5.5 to 10.0
Thermal stability*:
| Temperature | Activity |
|---|---|
| 40℃ or less | Stable |
| 50℃ | 95% active |
| 60℃ | 75% active |
| 70℃ | Almost inactive |
* Treated with 50 mmol/L phosphate buffer at pH 6.0 for 10 min
Optimal pH: 7.5 to 8.5
Optimal ionic strength: 10 mmol/L NaCl (in 10 mmol/L Tris-HCl buffer [pH 8.0])
Lytic spectrum
| Microorganism | Bacteriolytic activity* | |
|---|---|---|
| TBL-1(300U/mL） | Egg-white lysozyme (1,000 U/mL) | |
| Micrococcus caseolyticus | +++ | - |
| Micrococcus luteus | +++ | - |
| Pediococcus acidilactici | +++ | + |
| Staphylococcus aureus | +++ | ± |
| Microbacterium arborescens | ++ | + |
| Bacillus alvei | +++ | ± |
| Clostridium acetobutylicum | +++ | - |
| Brevibacterium leucinophagum | + | ± |
| Lactobacillus sake | +++ | + |
| Enterococcus feacalis | +++ | ± |
| Leuconostoc dextranicum | ++ | ± |
| Achromobacter liquidum | + | - |
| Nocardioides simplex | + | + |
| Beijerincka indica | + | ± |
| Micobacterium diernhoferi | ± | ± |
| Kurthia zopfii | ++ | + |
* Calculated from the decrease in absorbance in each microbial suspension at 600 nm
+++: decreased by ≥80% (within 10 min), ++: decreased by ≥80% (within 60 min), +: decreased by 80%–40% (within 60 min), ±: decreased by 40%–10% (within 60 min), -: decreased by 10%–0% (within 60 min).
Reference
- Isono, M., Takahashi, K. and Yamazaki, Y. : Patent Notice, Sho46-42953.
- Nakamura, K., Okazawa, Y., Soejima, M. and Masaki, T. : Agric. Biol. Chem., 37, 2667(1973).
- Horinouchi, S., Uozumi, T., Beppu, T. and Arima, K. : Agric. Biol. Chem., 41, 2487(1977).
- Kotani, S. : Protein, Nucleic Acid, Enzyme, 13, 1136(1968).
- Kotani, S. : Protein, Nucleic Acid, Enzyme, 14, 38(1969).
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For research use or further manufacturing use only. Not for use in diagnostic procedures.
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