Polyclonal antibodies for optic nerve research

4.1G, Mak, ICK, Trβ2 Polyclonal Antibodies

Polyclonal antibodies to factors involved in the development and function of optic nerves.

Features

  • Performances of each antibody has been demonstrated within KO mice
  • Applications are available

Antibody Information

Anti Mouse 4.1G / EPB41L2, Rabbit Anti Mouse ICK, Guinea Pig Anti Mouse Mak, Guinea Pig Anti Mouse Trβ2, Rabbit
Wako Cat. No. 018-26421 015-26431 012-26441 016-24261
Antigen Synthetic peptide
TPRLRKRGKDPSENRGIC
(57-73 a.a.) of
4.1G/EPB41L2
Synthetic peptide
corresponding to 346-412
a.a. of ICK
His-tagged C-terminal
sequence
(296-622 a.a.) of mouse Mak
Recombinant mouse Trβ2
N-terminal fragment
(1-107 a.a.)
Antigen
factor
information
4.1G (Alias: EPB41L2) is one of the ERM family proteins with a molecular weight about 113 kDa. It preserves the formation of cytoplasmic membranve. Recently, it is reported to regulate synaptic location in the retina and normal visuality by using KO mouse. It is also used as a rod cell marker. ICK (Alias: MRK, LCK2) is a kinase protein with a molecular weight about 71 kDa. It localizes at the tip of cilia regulating cliliary transport. ICK phosphorylates Kif3a and essential for proper cliliogenesis. Mak (Alias: DJ417M14.2) is a kinase protein with a molecular weight of 71 kDa. It regulates ciliary length by phosphorylating RP1. Trβ2 (Thyroid hormone receptor beta 2) is a high affinity receptor for triiodothyronine. Trβ2 is required for the development of green cone photoreceptors of embryonic retina. It is also used as a cone cell marker.
Host / Isotype Rabbit / IgG Guinea pig / IgG Guinea pig / IgG Rabbit / IgG
Species cross reactivity Mouse
(Other species have not been tested)
Mouse
(Other species have not been tested)
Mouse
(Other species have not been tested)
Mouse
(Other species have not been tested)
Applications ■Immunocytochemistry
 1 : 5,000 - 10,000
■Western Blot
 1 : 10,000 - 100,000
■Immunocytochemistry
 1 : 100
■Western Blot
 1 : 250
■Immunocytochemistry
 1 : 1,000
■Western Blot
 1 : 3,000
■Immunocytochemistry
 1 : 1,000
■Western Blot
 1 : 1,000
Application data
(Immuno-cytochemistry)
01216_img01.gif
Green:4.1G/EPB41L2
■Sample: Mouse retina
01216_img02.gif
Red:ICK
■Sample: Mouse embronic fibroblast
01216_img03.gif
Green:Mak Red:γ-tubulin
■Sample: Mouse retina
01216_img04.gif
Green:Trβ2
■Sample: Mouse retina
References Sanuki, R., et al.: Cell Reports, 10, 796 (2015). Chaya, T., et al.: The EMBO Journal, 33,1227 (2014). Omori, Y., et al.: Proc. Natl. Acad. Sci. 107, 22671 (2010). Sanuki, R., et al.: Nature Neurosci., 14, 1125 (2011).

Data was provided by Takahisa Furukawa, Laboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University.

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