Phos-tag™ Agarose Resins in Tip
Phos-tag™ Tip is a ready-to-use pipette-tip column prepacked with Phos-tag™ agarose. This column is a powerful tool for pretreating phosphorylated peptides.
The Phos-tag™ molecules in a pipette tip can specifically capture phosphorylated proteins in your samples. This allows for the easy purification of these proteins using only a pipetting operation.
- Short hands-on time (less than 30 min)
- High recovery yields
- No need for expensive equipment
Instructions for Use
Separation of Phosphopeptides from the Tryptic Digest of 6 nM β-Casein
- Following trypsin digestion of β-casein (6 nM), phosphorylated peptides were purified using a Phos-tag™ Tip column.
- Aliquots of the unpurified sample, flow-through fraction, and eluate were analyzed by HPLC.
We detected only phosphopeptide peaks (P1 and P2) in the eluate. This indicates that Phos-tag™ Tips can selectively trap and enrich phosphopeptides from the tryptic digest of β-casein.
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